Microbiology & Biotechnology Research Lab, Department of Biotechnology, Fatima Jinnah Women University, Rawalpindi, Pakistan.
J Environ Sci Health B. 2020;55(12):1106-1113. doi: 10.1080/03601234.2020.1823171. Epub 2020 Sep 29.
In the current scenario of overuse of pesticides (resulting in soil and water pollution and ultimately leading to biomagnification), a research project was carried out to study biodegradation of Triazophos. For this purpose, three bacterial strains ( MB490 MB498 and sp. MB504), isolated from cotton fields of Mianwali, Pakistan were investigated for Triazophos degradation and metabolite formation in M-9 broth, soil slurry and soil microcosm after incubation for 9 days. There was 88.4-95.8% Triazophos degradation in M-9 broth, 99.90% degradation in soil slurry and 92.74 to 96% Triazophos degradation in soil microcosm by these bacteria after 9 days. While there was negligible Triazophos degradation (upto 7%) in the controls without bacteria. According to GCMS analysis, 7 unique and novel metabolites (1, 2, 4-Triazole-4-amine, N-(2-Thienylmethyl), Benzene sulfonic acid hydrazide, Benzene sulfonic acid methyl ester, 4H-1,2,4-Triazole-4-benzenesulfonamide, 4, 5 dihydro-N-(O-toyl)-3-furamide, Ethyl 4-phenyldiazenylbenzoate and Dibutyl methanephosphonate) of Triazophos were revealed. Current results strongly suggest the potential of these bacterial strains for the remediation of Triazophos contaminated agricultural soils.
在当前农药过度使用的情况下（导致土壤和水污染，并最终导致生物放大），进行了一项研究三唑磷生物降解的研究项目。为此，从巴基斯坦木尔坦的棉田中分离出了三株细菌（MB490、MB498 和 sp.MB504），并在 M-9 肉汤、土壤泥浆和土壤微宇宙中进行了为期 9 天的培养，研究了它们对三唑磷的降解和代谢产物的形成。这些细菌在 M-9 肉汤中可实现 88.4-95.8%的三唑磷降解，在土壤泥浆中可实现 99.90%的降解，在土壤微宇宙中可实现 92.74%至 96%的三唑磷降解，而在没有细菌的对照中几乎没有三唑磷降解（最多 7%）。根据 GCMS 分析，发现了 7 种独特的新代谢物（1,2,4-三唑-4-胺、N-(2-噻吩甲基）苯磺酸酰肼、苯磺酸甲酯、4H-1,2,4-三唑-4-苯磺酰胺、4,5-二氢-N-(O- 特戊酰基）-3-糠酰胺、乙基 4-苯基亚氨基苯甲酸酯和二丁基甲膦酸酯）。目前的结果强烈表明这些细菌菌株具有修复三唑磷污染农田的潜力。
