用于检测去势抵抗性前列腺癌的单个循环肿瘤细胞的纳米力学生物标志物。
Nanomechanical biomarkers of single circulating tumor cells for detection of castration resistant prostate cancer.
机构信息
Department of Molecular Medicine, University of Texas Health Science Center, San Antonio, Texas.
出版信息
Prostate. 2014 Sep;74(13):1297-307. doi: 10.1002/pros.22846. Epub 2014 Jul 25.
BACKGROUND
Emerging evidence shows that nanomechanical phenotypes of circulating tumor cells (CTC) could become potential biomarkers for metastatic castration resistant prostate cancer (mCRPC).
METHODS
To determine the nanomechanical phenotypes of CTCs we applied atomic force microscopy (AFM) employing the PeakForce quantitative nanomechanical (QNM) imaging. We assessed biophysical parameters (elasticity, deformation, and adhesion) of 130 CTCs isolated from blood samples from five castration sensitive (CS) and 12 castration resistant prostate cancer (CRPCa) patients.
RESULTS
We found that CTCs from CRPCa patients are three times softer, three times more deformable, and seven times more adhesive than counterparts from CSPCa patients. Both nonsupervised hierarchical clustering and principle component analysis show that three combined nanomechanical parameters could constitute a valuable set to distinguish between CSPCa and CRPCa.
CONCLUSIONS
[corrected] Our study indicates that nanomechanical phenotypes of CTCs may serve as novel and effective biomarkers for mCRPC.
背景
新出现的证据表明，循环肿瘤细胞（CTC）的纳米力学表型可能成为转移性去势抵抗性前列腺癌（mCRPC）的潜在生物标志物。
方法
为了确定 CTC 的纳米力学表型，我们应用原子力显微镜（AFM）采用峰力定量纳米力学（QNM）成像。我们评估了从五例去势敏感（CS）和 12 例去势抵抗性前列腺癌（CRPCa）患者的血液样本中分离出的 130 个 CTC 的生物物理参数（弹性、变形和粘附）。
结果
我们发现，来自 CRPCa 患者的 CTC 比来自 CSPCa 患者的 CTC 柔软三倍，变形三倍，粘附性强七倍。无监督层次聚类和主成分分析均表明，三个联合纳米力学参数可构成区分 CSPCa 和 CRPCa 的有价值的集合。
结论
我们的研究表明，CTC 的纳米力学表型可能成为 mCRPC 的新型有效生物标志物。
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