Department of Virology 1, National Institute of Infectious Diseases, Japan.
Department of Virology 1, National Institute of Infectious Diseases, Japan.
J Infect Chemother. 2014 Oct;20(10):656-9. doi: 10.1016/j.jiac.2014.06.010. Epub 2014 Jul 25.
In this study, we investigated the prevalence of genital Chlamydia trachomatis isolated in Japan using a high-resolution genotyping method, the multilocus VNTR analysis (MLVA)-ompA typing method. Seventeen serotypes of C. trachomatis standard strain (A-L3) and 44 clinical isolates were obtained from clinical settings. Genotyping of the ompA gene allowed clinical isolates to be divided into nine serotypes: B (6.8%), D (15.9%), E (25%), F (20.5%), G (18.1%), H (6.8%), Ia (2.3%), J (2.3%), and K (2.3%). These isolates were further divided into 28 types after combining ompA genotyping data with MLVA data (Hunter-Gaston discriminatory index D, 0.949). Thus, our results demonstrated that MLVA could identify clinical isolates that could not be distinguished by ompA typing.
在这项研究中，我们使用高分辨率基因分型方法——多位点可变数目串联重复分析（MLVA）-ompA 分型方法，调查了在日本分离的生殖道沙眼衣原体的流行情况。从临床环境中获得了 17 种沙眼衣原体标准株（A-L3）和 44 种临床分离株。ompA 基因的基因分型使临床分离株分为 9 种血清型：B（6.8%）、D（15.9%）、E（25%）、F（20.5%）、G（18.1%）、H（6.8%）、Ia（2.3%）、J（2.3%）和 K（2.3%）。将 ompA 基因分型数据与 MLVA 数据结合后，这些分离株进一步分为 28 型（Hunter-Gaston 区分指数 D，0.949）。因此，我们的结果表明 MLVA 可以识别无法通过 ompA 分型区分的临床分离株。
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