Qin Xiaolin, Zheng Heping, Xue Yaohua, Ren Xuqi, Yang Bin, Huang Jinmei, Huang Shujie, Wu Xingzhong, Zeng Weiying, Ou Jiangli, Lan Yinyuan, Tang Sanmei
Guangdong Provincial Center for Skin Diseases and STIs Control and Prevention, Guangzhou, Guangdong, China.
Department of Laboratory Medicine, Southern Medical University, Guangzhou, Guangdong, China.
PLoS One. 2016 Jul 19;11(7):e0159658. doi: 10.1371/journal.pone.0159658. eCollection 2016.
Chlamydia trachomatis is one of the most prevalent bacterial sexually transmitted infection in China. Although C. trachomatis genotypes can be discriminated by outer membrane protein gene (ompA) sequencing, currently available methods have limited resolutions. This study used a high-resolution genotyping method, namely, multilocus variable number tandem-repeat analysis with ompA sequencing (MLVA)-ompA, to investigate the local epidemiology of C. trachomatis infections among men who have sex with men (MSM) and men who have sex with women (MSW) attending a sexually transmitted diseases (STD) clinic in Guangzhou, China.
Rectal specimens from MSM and urethral specimens from MSW were collected between January 2013 and July 2014 at the Guangdong Provincial Center STD clinic. The specimens were sent to the laboratory for analyses. All specimens that were tested positive for C. trachomatis by the commercial nucleic acid amplification tests were genotyped by MLVA-ompA.
Fifty-one rectal specimens from MSM and 96 urethral specimens from MSW were identified with C. trachomatis. One hundred and forty-four of the 147 specimens were fully genotyped by MLVA-ompA. Rectal specimens from MSM were divided into four ompA genotypes and urethral specimens from MSW into nine genotypes. No mixed infections were found among all specimens. The most frequent genotypes were D, G, J, E and F. All specimens were further divided into 46 types after ompA genotyping was combined with MLVA. Genotypes D-8.7.1 and G-3.4a.3 were the most frequent among MSM, whereas genotypes D-3.4a.4, E-8.5.1, F-8.5.1, and J-3.4a.2 were the most frequent subtypes among MSW. The discriminatory index D was 0.90 for MLVA, 0.85 for ompA, and 0.95 for MLVA-ompA.
The most prevalent MLVA-ompA genotypes were significantly different between MSM and MSW from Guangzhou, China. Moreover, MLVA-ompA represented a more favorable degree of discrimination than ompA and could be a reliable complement for ompA for the routine subtypes of C. trachomatis.
沙眼衣原体是中国最常见的细菌性性传播感染之一。虽然沙眼衣原体基因型可通过外膜蛋白基因（ompA）测序进行区分，但目前可用的方法分辨率有限。本研究采用一种高分辨率基因分型方法，即多基因座可变数目串联重复序列分析结合ompA测序（MLVA-ompA），来调查在中国广州一家性传播疾病（STD）诊所就诊的男男性行为者（MSM）和男女异性性行为者（MSW）中沙眼衣原体感染的当地流行病学情况。
2013年1月至2014年7月期间，在广东省STD中心诊所采集了MSM的直肠标本和MSW的尿道标本。标本被送往实验室进行分析。所有通过商业核酸扩增试验检测沙眼衣原体呈阳性的标本均采用MLVA-ompA进行基因分型。
在MSM的51份直肠标本和MSW的96份尿道标本中检测出沙眼衣原体。147份标本中的144份通过MLVA-ompA进行了完全基因分型。MSM的直肠标本分为4种ompA基因型，MSW的尿道标本分为9种基因型。所有标本中均未发现混合感染。最常见的基因型是D、G、J、E和F。ompA基因分型与MLVA相结合后，所有标本进一步分为46种类型。基因型D-8.7.1和G-3.4a.3在MSM中最常见，而基因型D-3.4a.4、E-8.5.1、F-8.5.1和J-3.4a.2在MSW中是最常见的亚型。MLVA的鉴别指数D为0.90，ompA为0.85，MLVA-ompA为0.95。
中国广州MSM和MSW中最常见的MLVA-ompA基因型存在显著差异。此外，MLVA-ompA比ompA具有更高的鉴别度，可作为ompA对沙眼衣原体常规亚型分型的可靠补充。
Zotero 插件