[源自小鼠和大鼠的肺支原体抗原对自然感染大鼠血清的反应性]
[The reactivity of antigens of Mycoplasma pulmonis derived from mice and rats to naturally infected rat sera].
作者信息
Terada E, Kobayashi N, Sakurai Y, Mochida R, Suzuki H, Takakura A, Kagiyama N
机构信息
Department of Laboratory Animal Science, School of Hygienic Sciences, Kitasato University, Sagamihara, Japan.
出版信息
Jikken Dobutsu. 1989 Jul;38(3):181-5. doi: 10.1538/expanim1978.38.3_181.
The reactivity of antigens of 4 mouse and 3 rat derived Mycoplasma pulmonis strains to 20 naturally infected rat sera was studied. The optical density values of the same serum by enzyme-linked immunosorbent assay using the 7 strains as the antigen revealed no marked difference among the strains. M. pulmonis antigens recognized by the antibodies were analyzed by the Western immunoblot method. The antigens with molecular weights of 92 K, 66 K, and 58 K were recognized in the 7 strains at a high frequency.
研究了4株源自小鼠和3株源自大鼠的肺炎支原体菌株的抗原对20份自然感染大鼠血清的反应性。以这7株菌株为抗原，通过酶联免疫吸附测定法检测同一血清的光密度值，结果显示各菌株之间无明显差异。采用Western免疫印迹法分析抗体识别的肺炎支原体抗原。在这7株菌株中，分子量为92K、66K和58K的抗原被高频识别。
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