肺支原体与关节炎支原体之间交叉反应性抗原的鉴定。

Minion F C, Brown M B, Cassell G H

Infect Immun. 1984 Jan;43(1):115-21. doi: 10.1128/iai.43.1.115-121.1984.

Serological cross-reactivity between Mycoplasma pulmonis and Mycoplasma arthritidis was investigated by enzyme-linked immunosorbent assay, immunoanalysis of electrophoretic blots, and protein A immunoprecipitation reactions. The results demonstrate that one-way cross-reactivity was present in both hyperimmunized and naturally infected rats and that the predominant cross-reactive antigens were M. pulmonis surface proteins. Distinct immunoblot patterns were demonstrated for M. pulmonis and M. arthritidis, allowing differentiation of the two species. The response to M. arthritidis antigens during natural infections differed greatly from that during hyperimmunization. Evidence suggested that nonprotein antigens were major determinants eliciting the antibody response to this mycoplasma.

通过酶联免疫吸附测定、电泳印迹免疫分析和蛋白A免疫沉淀反应，研究了肺支原体和关节炎支原体之间的血清学交叉反应性。结果表明，在高度免疫和自然感染的大鼠中均存在单向交叉反应性，且主要的交叉反应抗原是肺支原体表面蛋白。肺支原体和关节炎支原体呈现出不同的免疫印迹模式，从而可以区分这两个物种。自然感染期间对关节炎支原体抗原的反应与高度免疫期间的反应有很大差异。有证据表明，非蛋白抗原是引发针对这种支原体抗体反应的主要决定因素。