在 HeLa Hep2 细胞中辐射诱导有丝分裂灾难过程中基因表达的改变。
Alterations in gene expression during radiation-induced mitotic catastrophe in HeLa Hep2 cells.
机构信息
Department of Clinical Microbiology, Immunology, Umeå University, Umeå, Sweden.
Department of Radiation Sciences, Radiation Physics, Umeå University, Umeå, Sweden.
出版信息
Anticancer Res. 2014 Aug;34(8):3875-80.
AIM
To explore kinetic changes in the gene expression profile during radiation-induced mitotic catastrophes.
MATERIALS AND METHODS
Gene expression changes were measured in HPV-infected HeLa Hep2 tumor cells following exposure to 5 Gy of ionizing radiation ((60)Co). Signaling pathways were explored and correlated to the biological responses linked to mitotic catastrophe.
RESULTS
Following irradiation a transient G2-arrest was induced. Anaphase bridge formation and centrosome hyperamplification was observed. These phenotypical changes correlated well with the observed gene expression changes. Genes with altered expression were found to be involved in mitotic processes as well as G2- and spindle assembly checkpoints. Also centrosome-associated genes displayed an increased expression.
CONCLUSION
This study elucidates specific characteristics in the altered gene expression pattern induced by irradiation, which can be correlated to the events of mitotic catastrophe in HeLa Hep2 cells. Therapeutic strategies modulating these alterations might potentiate future therapy and enhance tumor cell killing.
目的
探讨辐射诱导有丝分裂灾难过程中基因表达谱的动态变化。
材料和方法
HPV 感染的 HeLa Hep2 肿瘤细胞在接受 5 Gy 电离辐射（（60）Co）后，测量基因表达的变化。探索信号通路，并将其与有丝分裂灾难相关的生物学反应相关联。
结果
照射后诱导短暂的 G2 期阻滞。观察到后期桥形成和中心体过度扩增。这些表型变化与观察到的基因表达变化很好地相关。表达发生改变的基因参与有丝分裂过程以及 G2 和纺锤体组装检查点。此外，中心体相关基因的表达也增加。
结论
本研究阐明了照射诱导的基因表达模式的特定特征，这些特征可与 HeLa Hep2 细胞有丝分裂灾难的事件相关联。调节这些改变的治疗策略可能增强未来的治疗效果并增强肿瘤细胞杀伤。
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