平卧漆姑草地上部分提取物的抗氧化活性和酚类含量。

Vasincu Al, Paulsen Berit Smestad, Diallo D, Bild Veronica, Miron Anca

Rev Med Chir Soc Med Nat Iasi. 2014 Apr-Jun;118(2):564-70.

UNLABELLED

In Malian traditional medicine, aerial parts of Glinus oppositifolius are used in the treatment of many disorders, particularly inflammations and joint pains.

AIM

The present study was initiated to investigate the antioxidant potential of Glinus oppositifolius.

MATERIAL AND METHODS

Aqueous and ethanol extracts were prepared from the dried aerial parts. Both extracts were examined for the phenolic content and antioxidant activity. Total phenolic content was quantified by Folin-Ciocalteu method. Antioxidant activity was studied in vitro by evaluating the ability to scavenge different free radicals (ABTS, superoxide and hydroxyl radicals, nitric oxide) and to chelate ferrous ions.

RESULTS

Aqueous extract was found to contain a higher level of total phenols than ethanol extract (1.27 +/- 0.04 vs. 0.94 +/- 0.05 g GAE/100 g extract). Both extracts lacked the ability to scavenge superoxide anion radical but scavenged ABTS and hydroxyl radicals, nitric oxide and chelated ferrous ions in a concentration-dependent manner. According to the EC50 values, aqueous extract showed stronger antioxidant effects than ethanol extract (174.32 +/- 1.49 vs. 276.76 +/- 1.52 microg/mL in ABTS radical cation scavenging assay, 119.91 +/- 1.70 vs. 240.57 +/- 0.97 microg/mL in nitric oxide scavenging assay, 1.99 +/- 0.01 vs. 6.54 +/- 0.08 mg/mL in ferrous ion chelating assay). In hydroxyl radical scavenging assay, aqueous extract had an EC50 value of 0.86 +/- 0.00 mg/mL while the EC50 value of ethanol extract was higher than the highest concentration that was tested (3.75 mg/mL).

CONCLUSIONS

Our results clearly indicate the antioxidant potential of Glinus oppositifolius aerial parts and suggest that the antiinflammatory activity might be partly related to the antioxidant potential.

未标注

在马里传统医学中，对叶金午时花的地上部分被用于治疗多种疾病，尤其是炎症和关节疼痛。

目的

开展本研究以调查对叶金午时花的抗氧化潜力。

材料与方法

从干燥的地上部分制备水提取物和乙醇提取物。对两种提取物进行酚类含量和抗氧化活性检测。总酚含量采用福林-西奥尔特法进行定量。通过评估清除不同自由基（ABTS、超氧阴离子和羟基自由基、一氧化氮）以及螯合亚铁离子的能力，在体外研究抗氧化活性。

结果

发现水提取物中的总酚含量高于乙醇提取物（1.27±0.04对0.94±0.05 g没食子酸当量/100 g提取物）。两种提取物均缺乏清除超氧阴离子自由基的能力，但能清除ABTS和羟基自由基、一氧化氮，并以浓度依赖的方式螯合亚铁离子。根据半数有效浓度（EC50）值，水提取物显示出比乙醇提取物更强的抗氧化作用（在ABTS自由基阳离子清除试验中为174.32±1.49对276.76±1.52 μg/mL，在一氧化氮清除试验中为119.91±1.70对240.57±0.97 μg/mL，在亚铁离子螯合试验中为1.99±0.01对6.54±0.08 mg/mL）。在羟基自由基清除试验中，水提取物的EC50值为0.86±0.00 mg/mL，而乙醇提取物的EC50值高于所测试的最高浓度（3.75 mg/mL）。