Division of Systematic Forest and Forest Products Sciences, Department of agro-environmental sciences, Faculty of Agriculture, Graduate School of Kyushu University, 812-8581, Japan.
J Ethnopharmacol. 2013 Jul 30;148(3):980-7. doi: 10.1016/j.jep.2013.06.008. Epub 2013 Jun 12.
Angelica shikokiana has been used as a health food for its anticancer, anti-inflammatory, antibacterial, antiallergic, and blood vessel dilating effects in Japan. It can also be used to prevent and treat hepatitis, diabetes, hyperlipidemia, and arteriosclerosis.
The present study was designed to compare the biological activities such as melanin synthesis inhibitory, anti-allergy, anti-lipase, anti-bacterial, anti-oxidant, and neuroprotective activities of different parts of the plant that may justify the use of this plant in folk medicine.
The roots, stems, leaves and, seeds of Angelica shikokiana were separately extracted with water and ethanol. Each extract was examined for melanin synthesis inhibitory and anti-allergy activity on B16-melanoma and RBL-2H3 cells using IgE and A23187 as a stimulant for β-hexosaminidase release, respectively. We also evaluated the inhibition of two enzymes, lipase and acetylcholine esterase, and of the bacterial growth of two species, Escherichia coli and Staphylococcus aureaus. The anti-oxidant activity was determined using oxygen radical anti-oxidant capacity, ORAC assay and its relation to the phenolic content was estimated using the Folin-Ciocalteu method. Besides, the protective effect of the extracts against H2O2-induced oxidative stress in mouse neuroblastoma, Neuro-2A cells was investigated.
The most active extract exhibiting melanin synthesis inhibition (63%) and at the same time with low cytotoxicity (15%) was the ethanol extract of roots at 20 µg/ml, followed by the ethanol extract of stems (57% inhibition, 5% cytotoxicity). On the other hand, the highest inhibitions of β-hexosaminidase release were recorded for the ethanol extract of leaves with IC50 value of 6.89 µg/ml followed by the water extract of the seeds and leaves with IC50 value of 78.32 and 88.44 µg/ml, respectively. For anti-lipase assay, ethanol extracts of the stems and roots showed the strongest inhibition with IC50 values of 204.06 and 216.24 µg/ml, respectively. None of the examined extracts showed any activity against Escherichia coli. while the ethanol extract of the roots and stems showed moderate inhibition for Staphylococcus aureus with minimum inhibitory concentration of 400 µg/ml. Ethanol extract of the roots showed only 30% inhibition of acetylcholine esterase enzyme. The results of anti-oxidant, phenolic content and protective effect against H2O2-induced cytotoxicity assays showed highly correlated data. Ethanol extract of the stems (ORAC value of 1.08 µmol Trolox/mg and phenolic content 44.25 μg GAE/mg) increased the cell viability of H2O2-treated Neuro-2A cells by 28%.
在日本，当作为具有抗癌、抗炎、抗菌、抗敏和血管扩张作用的保健品时，当归被使用。它还可以用于预防和治疗肝炎、糖尿病、高血脂和动脉硬化。
本研究旨在比较植物不同部位的生物活性，如黑色素合成抑制、抗过敏、抗脂肪酶、抗菌、抗氧化和神经保护活性，以证明这种植物在民间医学中的应用。
当归的根、茎、叶和种子分别用水和乙醇提取。使用 IgE 和 A23187 作为 β-己糖胺酶释放的刺激物，在 B16-黑色素瘤和 RBL-2H3 细胞上分别检查每种提取物的黑色素合成抑制和抗过敏活性。我们还评估了两种酶，脂肪酶和乙酰胆碱酯酶的抑制作用，以及两种细菌，大肠杆菌和金黄色葡萄球菌的生长抑制作用。使用氧自由基抗氧化能力（ORAC）测定法评估抗氧化活性，并使用 Folin-Ciocalteu 法估计其与酚含量的关系。此外，还研究了提取物对 H2O2 诱导的小鼠神经母细胞瘤 Neuro-2A 细胞氧化应激的保护作用。
在 20μg/ml 时，显示黑色素合成抑制（63%）和低细胞毒性（15%）的最活跃提取物是根的乙醇提取物，其次是茎的乙醇提取物（57%抑制，5%细胞毒性）。另一方面，记录到β-己糖胺酶释放的最高抑制作用是叶的乙醇提取物，IC50 值为 6.89μg/ml，其次是种子和叶的水提取物，IC50 值分别为 78.32μg/ml 和 88.44μg/ml。对于抗脂肪酶测定，茎和根的乙醇提取物显示出最强的抑制作用，IC50 值分别为 204.06μg/ml 和 216.24μg/ml。检查的提取物均对大肠杆菌无任何活性，而根和茎的乙醇提取物对金黄色葡萄球菌显示出中等抑制作用，最低抑菌浓度为 400μg/ml。根的乙醇提取物仅对乙酰胆碱酯酶表现出 30%的抑制作用。抗氧化、酚含量和对 H2O2 诱导的细胞毒性的保护作用测定的结果显示出高度相关的数据。茎的乙醇提取物（ORAC 值为 1.08μmol Trolox/mg 和酚含量为 44.25μg GAE/mg）使 H2O2 处理的 Neuro-2A 细胞的细胞活力增加了 28%。
