Roughley P J, Hatt M, Mason R M
Biochim Biophys Acta. 1978 Apr 3;539(4):445-58. doi: 10.1016/0304-4165(78)90078-8.
Bovine nasal cartilage was extracted with 0.5 M LaCl3 and the extract then diluted with nine volumes of water. The resulting precipitated (PLaCl3) contained the proteoglycan subunits, together with minor protein components, but was essentially free from hyaluronic acid. The properties of PLaCl3 were investigated by chemical analysis, electrophoresis, viscometry and analytical ultracentrifugation, and the results compared with those for proteoglycan obtained by caesium chloride density gradient centrifugation of 2 M CaCl2 cartilage extracts. Proteoglycan subunits (A1D1) prepared from PLaCl3 showed identical properties to those obtained from other high ionic strength cartilage extracts.
用0.5M的LaCl₃提取牛鼻软骨，然后将提取物用九倍体积的水稀释。所得沉淀物（PLaCl₃）含有蛋白聚糖亚基以及少量蛋白质成分，但基本不含透明质酸。通过化学分析、电泳、粘度测定和分析超速离心研究了PLaCl₃的性质，并将结果与通过对2M CaCl₂软骨提取物进行氯化铯密度梯度离心获得的蛋白聚糖的结果进行了比较。由PLaCl₃制备的蛋白聚糖亚基（A1D1）显示出与从其他高离子强度软骨提取物中获得的亚基相同的性质。
