• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
The composition of cartilage proteoglycans. An investigation using high- and low-inonic-strength extraction procedures.软骨蛋白聚糖的组成。一项使用高离子强度和低离子强度提取程序的研究。
Biochem J. 1973 Mar;131(3):541-53. doi: 10.1042/bj1310541.
2
The electrophoretic heterogeneity of bovine nasal cartilage proteoglycans.牛鼻软骨蛋白聚糖的电泳异质性
Biochem J. 1976 Aug 1;157(2):357-67. doi: 10.1042/bj1570357.
3
Proteoglycan aggregates in adult human costal cartilage.成人肋软骨中的蛋白聚糖聚集体。
Biochim Biophys Acta. 1979 Apr 3;583(4):512-26. doi: 10.1016/0304-4165(79)90068-0.
4
The nature of the protein moieties of cartilage proteoglycans of pig and ox.猪和牛软骨蛋白聚糖中蛋白质部分的性质。
Biochem J. 1975 Sep;149(3):657-68. doi: 10.1042/bj1490657.
5
Studies on protein-polysaccharides from pig laryngeal cartilage. Heterogeneity, fractionation and characterization.猪喉软骨中蛋白质多糖的研究。异质性、分级分离与特性分析。
Biochem J. 1969 Aug;113(5):885-94. doi: 10.1042/bj1130885.
6
Hyaluronic acid in cartilage and proteoglycan aggregation.软骨中的透明质酸与蛋白聚糖聚集
Biochem J. 1974 Jun;139(3):565-81. doi: 10.1042/bj1390565.
7
The proteoglycans of the human intervertebral disc.人类椎间盘的蛋白聚糖
Biochem J. 1975 Mar;145(3):549-56. doi: 10.1042/bj1450549.
8
Studies on the polydispersity and heterogeneity of cartilage proteoglycans. Identification of 3 proteoglycan structures in bovine nasal cartilage.软骨蛋白聚糖的多分散性和异质性研究。牛鼻软骨中3种蛋白聚糖结构的鉴定。
Biochem J. 1975 Dec;151(3):581-94. doi: 10.1042/bj1510581.
9
Glycosaminoglycans and proteoglycans of human chondrosarcoma.人类软骨肉瘤的糖胺聚糖和蛋白聚糖
Biochim Biophys Acta. 1976 Jun 23;437(1):71-86. doi: 10.1016/0304-4165(76)90348-2.
10
Characteristics of the protein-keratan sulfate core and of keratan sulfate prepared from bovine nasal cartilage proteoglycan.从牛鼻软骨蛋白聚糖制备的蛋白质-硫酸角质素核心及硫酸角质素的特性
J Biol Chem. 1972 Jul 25;247(14):4529-38.

引用本文的文献

1
Studies on a stable, mild diabetes induced by streptozotocin in rats.链脲佐菌素诱导大鼠稳定、轻度糖尿病的研究。
Br J Exp Pathol. 1982 Aug;63(4):408-13.
2
Chemical and immunochemical characterization of caseins and the major whey proteins of rabbit milk.兔乳中酪蛋白和主要乳清蛋白的化学及免疫化学特性
Biochem J. 1982 Jan 1;201(1):71-9. doi: 10.1042/bj2010071.
3
Hyaluronic acid in cartilage and proteoglycan aggregation.软骨中的透明质酸与蛋白聚糖聚集
Biochem J. 1974 Jun;139(3):565-81. doi: 10.1042/bj1390565.
4
Extraction of cartilage protein-polysaccharides with inorganic salt solutions.用无机盐溶液提取软骨蛋白多糖。
Biochem J. 1973 Mar;131(3):535-40. doi: 10.1042/bj1310535.
5
Bone morphogenetic protein: a review.
Int Orthop. 1991;15(2):169-77. doi: 10.1007/BF00179720.
6
Insoluble collagen of methylcholanthrene induced sarcoma.
Mol Cell Biochem. 1975 Jul 31;8(1):23-30. doi: 10.1007/BF01731646.
7
Purification of the glycoprotein lectin from the broad bean (Vicia faba) and a comparison of its properties with lectins of similar specificity.从蚕豆（Vicia faba）中纯化糖蛋白凝集素及其与具有相似特异性的凝集素的性质比较。
Biochem J. 1976 Apr 1;155(1):127-35. doi: 10.1042/bj1550127.
8
A comparative study of the proteoglycan of growth cartilage of normal and rachitic chicks.正常雏鸡与佝偻病雏鸡生长软骨蛋白聚糖的比较研究。
Biochem J. 1978 Jun 1;171(3):675-82. doi: 10.1042/bj1710675.
9
The degradation of cartilage proteoglycans by tissue proteinases. Proteoglycan structure and its susceptibility to proteolysis.组织蛋白酶对软骨蛋白聚糖的降解。蛋白聚糖结构及其对蛋白水解的敏感性。
Biochem J. 1977 Dec 1;167(3):629-37. doi: 10.1042/bj1670629.
10
A lectin from the exudate of the fruit of the vegetable marrow (Cucurbita pepo) that has a specificity for beta-1,4-linked N-acetylglucosamine oligosaccharides.一种来自西葫芦（南瓜属西葫芦）果实渗出物的凝集素，它对β-1,4-连接的N-乙酰葡糖胺寡糖具有特异性。
Biochem J. 1979 Oct 1;183(1):133-7. doi: 10.1042/bj1830133.

本文引用的文献

1
THE USE OF LANTHANUM TO STUDY THE DEGRADATION OF A PROTEINPOLYSACCHARIDE FROM CARTILAGE.利用镧研究软骨中一种蛋白多糖的降解
J Biol Chem. 1964 May;239:1498-503.
2
A modified uronic acid carbazole reaction.一种改良的糖醛酸咔唑反应。
Anal Biochem. 1962 Oct;4:330-4. doi: 10.1016/0003-2697(62)90095-7.
3
Structure of connective tissues, a chemical point of view.结缔组织的结构：化学视角
Fed Proc. 1966 May-Jun;25(3):1047-52.
4
The separation of new forms of the proteinpolysaccharides of bovine nasal cartilage.牛鼻软骨蛋白多糖新形式的分离
J Biol Chem. 1966 Sep 25;241(18):4261-6.
5
Proteinpolysaccharide complex from bovine nasal cartilage. The function of glycoprotein in the formation of aggregates.来自牛鼻软骨的蛋白多糖复合物。糖蛋白在聚集体形成中的作用。
J Biol Chem. 1969 May 10;244(9):2384-96.
6
Mobility measurements by photometric analysis of zone electrophoresis in a sucrose gradient column.通过在蔗糖梯度柱中进行区带电泳的光度分析来进行迁移率测量。
Anal Biochem. 1968 Oct 24;25(1):30-9. doi: 10.1016/0003-2697(68)90077-8.
7
Protein-polysaccharide complexes from adult human tracheal cartilage.来自成年人类气管软骨的蛋白质-多糖复合物。
Biochem J. 1970 Sep;119(3):599-601. doi: 10.1042/bj1190599.
8
Physical properties and polydispersity of proteoglycan from bovine nasal cartilage.来自牛鼻软骨的蛋白聚糖的物理性质和多分散性
J Biol Chem. 1970 Oct 10;245(19):4920-30.
9
Sequence of bovine liver glutamate dehydrogenase. I. Isolation of tryptic peptides from the carboxymethylated protein.牛肝谷氨酸脱氢酶的序列。I. 从羧甲基化蛋白中分离胰蛋白酶肽段。
J Biol Chem. 1971 Apr 25;246(8):2360-73.
10
Isolation of the peptide core of costal cartilage chondroitin 4-sulfate proteoglycan.肋软骨硫酸软骨素4-硫酸酯蛋白聚糖肽核心的分离
Biochemistry. 1972 May 9;11(10):1856-61. doi: 10.1021/bi00760a020.

软骨蛋白聚糖的组成。一项使用高离子强度和低离子强度提取程序的研究。

The composition of cartilage proteoglycans. An investigation using high- and low-inonic-strength extraction procedures.

作者信息

Mayes R W, Mason R M, Griffin D C

出版信息

Biochem J. 1973 Mar;131(3):541-53. doi: 10.1042/bj1310541.

DOI:10.1042/bj1310541
PMID:4269049
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177501/
Abstract
  1. A proteoglycan fraction (the proteoglycan subunit fraction) was prepared from extracts, with 0.15m-KCl (low-ionic-strength) and 0.5m-LaCl(3), 2.0m-CaCl(2) and 4.0m-guanidinium chloride (high-ionic-strength), of bovine nasal cartilage by equilibrium-density-gradient centrifugation, essentially as described by Hascall & Sajdera (1969). 2. The use of different centrifugation times showed that near-equilibrium conditions were reached by 48h for the fractions prepared from the high-ionic-strength extracts. The fraction isolated from the low-ionic-strength extract required a longer centrifugation time to reach equilibrium conditions. 3. The composition of the proteoglycan fractions from the various extracts was compared by analyses of their carbohydrate and amino acid contents. Difference indices were calculated from the amino acid analysis to compare the degree of compositional relationship between the protein components of the proteoglycans. 4. Small compositional differences were found between the proteoglycans isolated from the various high-ionic-strength extracts. The protein content of the fractions from the CaCl(2) extract and the guanidinium chloride extract showed the greatest difference in this respect, although their amino acid analysis was similar. 5. The proteoglycan fraction isolated from the low-ionic-strength extract shows marked differences in composition from the fractions isolated from the high-ionic-strength extracts. Its protein and glucosamine contents were lower whereas its hexuronic acid and galactosamine contents were higher than those of the latter. It also exhibits major differences in its amino acid composition. The glucosamine:galactosamine ratio of the fraction from the low-ionic-strength extract indicates that it may be an almost exclusively chondroitin sulphate-proteoglycan. Its analysis correlates closely with that of a low-molecular-weight proteoglycan isolated from pig laryngeal cartilage by Tsiganos & Muir (1969). 6. The proteoglycan fractions from both the low- and high-ionic-strength extracts migrate as a single band in zone electrophoresis carried out in a sucrose-density gradient at both pH3.0 and pH7.0, although each showed evidence of band widening during the electrophoresis. All the proteoglycan fractions migrated with the same electrophoretic mobility at pH3.0, irrespective of the differences in composition between them. 7. The differences between the proteoglycans from the low- and high-ionic-strength extracts are discussed and the view is advanced that they may be due to association between predominantly chondroitin sulphate-proteoglycans and a keratan sulphate-enriched proteoglycan species.
摘要

  1. 按照哈斯卡尔和萨伊德拉（1969年）所述的基本方法，通过平衡密度梯度离心法，从牛鼻软骨提取物中制备蛋白聚糖组分（蛋白聚糖亚基组分），该提取物含有0.15m - KCl（低离子强度）以及0.5m - LaCl₃、2.0m - CaCl₂和4.0m - 氯化胍（高离子强度）。

  2. 不同离心时间的使用表明，对于从高离子强度提取物制备的组分，48小时可达到接近平衡的条件。从低离子强度提取物中分离的组分需要更长的离心时间才能达到平衡条件。

  3. 通过分析各种提取物中蛋白聚糖组分的碳水化合物和氨基酸含量，对其组成进行比较。根据氨基酸分析计算差异指数，以比较蛋白聚糖蛋白质组分之间的组成关系程度。

  4. 在从各种高离子强度提取物中分离出的蛋白聚糖之间发现了微小的组成差异。在这方面，CaCl₂提取物和氯化胍提取物的组分蛋白质含量差异最大，尽管它们的氨基酸分析相似。

  5. 从低离子强度提取物中分离出的蛋白聚糖组分与从高离子强度提取物中分离出的组分在组成上有明显差异。其蛋白质和氨基葡萄糖含量较低，而己糖醛酸和半乳糖胺含量高于后者。其氨基酸组成也存在主要差异。低离子强度提取物组分的氨基葡萄糖：半乳糖胺比率表明它可能几乎完全是硫酸软骨素蛋白聚糖。其分析与齐加诺斯和缪尔（1969年）从猪喉软骨中分离出的低分子量蛋白聚糖的分析密切相关。

  6. 在pH3.0和pH7.0的蔗糖密度梯度区带电泳中，低离子强度和高离子强度提取物中的蛋白聚糖组分均以单一谱带迁移，尽管在电泳过程中每条谱带都有展宽的迹象。在pH3.0时，所有蛋白聚糖组分均以相同的电泳迁移率迁移，无论它们之间的组成差异如何。

  7. 讨论了低离子强度和高离子强度提取物中蛋白聚糖之间的差异，并提出观点认为它们可能是由于主要的硫酸软骨素蛋白聚糖与富含硫酸角质素的蛋白聚糖种类之间的缔合所致。