Nanda Tarun, Jain Sanjeev, Kaur Harjit, Kapoor Daljit, Nanda Sonia, Jain Rohit
Department of Periodontology, Post Graduate Institute of Dental Sciences, Rohtak, Haryana, India.
Department of Periodontology and Oral Implantology, Guru Nanak Dev Dental College and Research Institute, Sunam, Punjab, India.
J Nat Sci Biol Med. 2014 Jul;5(2):356-8. doi: 10.4103/0976-9668.136183.
Root surfaces of periodontitis-affected teeth are hypermineralized and contaminated with cytotoxic and other biologically active substances. To achieve complete decontamination of the tooth surfaces, various methods including root conditioning following scaling and root planning are present. The main objective of this article is to throw light on the different root conditioning agents used and the goals accomplished by root conditioning in the field of periodontology.
20 human maxillary anterior teeth indicated for extraction due to chronic periodontitis were collected and root planned. The teeth were sectioned and specimens were divided into two groups - Group I and II. Group I dentin specimens were treated with EDTA and group II specimens were treated with tetracycline HCl solution at concentration of 10% by active burnishing technique for 3 minutes. The root surface samples were then examined by scanning electron microscope (SEM).
The results of the study showed that EDTA and tetracycline HCl were equally effective in removing the smear layer. It was observed that the total and patent dentinal tubules were more in number in teeth treated with tetracycline as compared to EDTA group. However, EDTA was found to be much more effective as root conditioning agent because it enlarged the diameter of dentinal tubules more than that of tetracycline HCl.
Results of in-vitro study showed that both the agents are good root conditioning agents if applied in addition to periodontal therapy. However, further studies are required to establish the in-vivo importance of EDTA and tetracycline HCL as root conditioners.
牙周炎患牙的牙根表面过度矿化,并被细胞毒性及其他生物活性物质污染。为实现牙面的彻底清洁,存在多种方法,包括在龈下刮治和根面平整后进行根面处理。本文的主要目的是阐明牙周病学领域中使用的不同根面处理剂以及根面处理所达成的目标。
收集20颗因慢性牙周炎而需拔除的人类上颌前牙,并进行根面平整。将牙齿切片,标本分为两组——第一组和第二组。第一组牙本质标本用乙二胺四乙酸(EDTA)处理,第二组标本用浓度为10%的盐酸四环素溶液通过主动抛光技术处理3分钟。然后通过扫描电子显微镜(SEM)检查根面样本。
研究结果表明,EDTA和盐酸四环素在去除玷污层方面同样有效。观察到,与EDTA组相比,用四环素处理的牙齿中总的和开放的牙本质小管数量更多。然而,发现EDTA作为根面处理剂更有效,因为它使牙本质小管的直径比盐酸四环素扩大得更多。
体外研究结果表明,如果在牙周治疗之外应用,这两种药剂都是良好的根面处理剂。然而,需要进一步研究来确定EDTA和盐酸四环素作为根面处理剂在体内的重要性。