Kirchhoff A L, Viapiana R, Miranda C E S, Sousa Neto M D, Cruz Filho A M
School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil.
Indian J Dent Res. 2014 May-Jun;25(3):370-4. doi: 10.4103/0970-9290.138341.
The action of endodontic instruments during biomechanical preparation results in smear layer formation. Removing the smear layer enhances disinfection into dentinal tubules in addition to allowing tridimensional sealing of the root canal system.
This study was designed to evaluate the smear layer removal and quantify the calcium ion release resulting from final irrigation with different chelating solutions.
Fifty human canines were instrumented and the final irrigation was performed with apple vinegar, 5% malic acid, 5% acetic acid, 17% ethylenediamine tetra-acetic acid (EDTA), and distilled water (control), which were collected and analyzed by atomic absorption spectrometry to quantify the concentration of calcium ions released. Smear layer removal was assessed in the cervical, middle, and apical thirds by SEM.
There was statistically significant difference (P < 0.001) between 17% EDTA and the other solutions with regard to smear layer removal. Apple vinegar, 5% malic acid, and 5% acetic acid promoted similar root canal cleaning. There was no statistical difference among the root canal thirds. The highest concentrations of calcium ions were obtained with 17% EDTA (P<0.001) followed by malic acid, apple vinegar, and acetic acid. Apple vinegar and acetic acid removed the smallest quantity of calcium ions.
It was concluded that 17% EDTA enabled greater smear layer removal and promoted release of the highest concentrations of calcium ions than the other solutions tested.
在生物机械预备过程中,根管器械的操作会导致玷污层的形成。去除玷污层除了能实现根管系统的三维封闭外,还能增强对牙本质小管的消毒作用。
本研究旨在评估玷污层的去除情况,并对用不同螯合溶液进行最终冲洗后钙离子的释放量进行量化。
选取50颗人犬齿进行器械预备,并用苹果醋、5%苹果酸、5%乙酸、17%乙二胺四乙酸(EDTA)和蒸馏水(对照)进行最终冲洗,收集冲洗液并用原子吸收光谱法分析,以量化释放的钙离子浓度。通过扫描电子显微镜在牙颈部、中部和根尖三分之一处评估玷污层的去除情况。
在玷污层去除方面,17% EDTA与其他溶液之间存在统计学显著差异(P < 0.001)。苹果醋、5%苹果酸和5%乙酸促进的根管清洁效果相似。根管的三个部分之间没有统计学差异。17% EDTA释放的钙离子浓度最高(P<0.001),其次是苹果酸、苹果醋和乙酸。苹果醋和乙酸去除的钙离子量最少。
得出的结论是,与其他测试溶液相比,17% EDTA能更有效地去除玷污层,并促进释放最高浓度的钙离子。