5%碳水化合物衍生黄腐酸对清除玷污层和根管牙本质显微硬度的影响——一项体外研究。

Effect of 5% Carbohydrate Derived-Fulvic Acid on Smear Layer Removal and Root Dentin Microhardness - An In Vitro study.

机构信息

Department of Conservative Dentistry and Endodontics, SRM Institute of Science and Technology, SRM Dental College, Ramapuram Campus, Chennai, India.

出版信息

Eur Endod J. 2022 Jun;7(2):156-160. doi: 10.14744/eej.2022.97759.

DOI:10.14744/eej.2022.97759

PMID:35786580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9285997/

Abstract

OBJECTIVE

To compare the ability of 5% carbohydrate derived-fulvic acid (CHD-FA) with 17%ethylenediamine tetraaceticacid (EDTA) on smear layer removal when used as a final irrigant, and to assess their effect on root dentin microhardness.

METHODS

A total of 56 single-rooted human mandibular premolars were decoronated to obtain standardized root length of 12 mm. Canal patency was established using 10 size K-file, working length was determined. Based on the irrigation regimen, samples were assigned into three groups; 17% EDTA, 5% CHD-FA and distilled water (control). The canals were instrumented with Protaper till F3 size. A standardized volume of 5 mL of each testing solution was used for 1 min. Roots were longitudinally split into two halves with one half of the samples for SEM analysis to evaluate the smear layer removal at coronal, middle, and apical thirds of root dentin. The other halves of the samples were subjected to Vickers microhardness testing. The data was statistically analyzed using Kruskal Wallis and Post Hoc test (P<0.05).

RESULTS

5% CHD-FA and 17% EDTA showed significant smear layer removal at all levels compared to distilled water (P<0.05). In the apical third, 5% CHD-FA showed significantly increased smear layer removal than EDTA (P<0.05). Microhardness testing of apical third showed significantly lesser reduction in microhardness for CHD-FA than EDTA (P<0.05).

CONCLUSION

5% CHD-FA could be a promising final irrigant for smear layer removal with decreased microhardness reduction on root dentin compared to 17% EDTA.

摘要

目的

比较 5%碳水化合物衍生富啡酸（CHD-FA）和 17%乙二胺四乙酸（EDTA）作为终末冲洗液去除玷污层的能力，并评估它们对根管牙本质显微硬度的影响。

方法

共 56 颗下颌前磨牙进行去冠处理，获得标准化的 12mm 根长。使用 10 号 K 锉建立根管通畅性，确定工作长度。根据冲洗方案，将样本分为三组；17%EDTA、5%CHD-FA 和蒸馏水（对照组）。用 Protaper 锉从 F3 号开始根管预备。每种测试溶液使用 5mL 的标准化体积进行 1 分钟冲洗。将根部分为两半，一半用于 SEM 分析，以评估牙本质冠、中、根尖三分之一处的玷污层去除情况。另一半样本进行维氏显微硬度测试。使用 Kruskal Wallis 和 Post Hoc 检验（P<0.05）对数据进行统计学分析。

结果

与蒸馏水相比，5%CHD-FA 和 17%EDTA 在所有水平均显示出显著的玷污层去除效果（P<0.05）。在根尖三分之一处，5%CHD-FA 的玷污层去除效果明显优于 EDTA（P<0.05）。根尖三分之一处的显微硬度测试显示，CHD-FA 对牙本质显微硬度的降低明显小于 EDTA（P<0.05）。