对杂交大丽花品种转录组进行测序和从头组装。
Sequencing and de novo assembly of a Dahlia hybrid cultivar transcriptome.
机构信息
Department of Genetics, Stanford University School of Medicine Stanford, CA, USA ; Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine Madison, WI, USA.
Department of Biology, Stanford University Stanford, CA, USA.
出版信息
Front Plant Sci. 2014 Jul 17;5:340. doi: 10.3389/fpls.2014.00340. eCollection 2014.
Dahlia variabilis, with an exceptionally high diversity of floral forms and colors, is a popular flower amongst both commercial growers and hobbyists. Recently, some genetic controls of pigment patterns have been elucidated. These studies have been limited, however, by the lack of comprehensive transcriptomic resources for this species. Here we report the sequencing, assembly, and annotation of the transcriptome of the developing leaves, stems, and floral buds of D. variabilis. This resulted in 35,638 contigs, most of which seem to contain the complete coding sequence, and of which 20,881 could be successfully annotated by similarity to UniProt. Furthermore, we conducted a preliminary investigation to identify contigs with expression patterns consistent with tissue-specificity. These results will accelerate research into the genetic controls of pigmentation and floral form of D. variabilis.
大丽花变异品种拥有极其多样化的花卉形态和颜色，在商业种植者和花卉爱好者中都很受欢迎。最近，一些色素模式的遗传控制已经被阐明。然而，这些研究受到该物种缺乏全面转录组资源的限制。在这里，我们报告了大丽花发育叶片、茎和花蕾的转录组测序、组装和注释。这产生了 35638 个连续序列，其中大多数似乎包含完整的编码序列，并且其中 20881 个可以通过与 UniProt 的相似性成功注释。此外，我们还进行了一项初步研究，以鉴定与组织特异性表达模式一致的连续序列。这些结果将加速对大丽花色素和花卉形态遗传控制的研究。
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