Conn M I, Kueppers F, Deren J, Abrams W, Weinbaum G
Department of Medicine, Graduate Hospital, Philadelphia, Pennsylvania.
Pancreas. 1989;4(6):724-32. doi: 10.1097/00006676-198912000-00011.
Experimental pancreatitis can be induced by an ethionine-containing, choline-deficient diet in mice. We investigated the role of circulating alpha 1-antitrypsin in this model using two strains of mice: ICR and C57BL-6. A 50% reduction in circulating alpha 1-antitrypsin occurred in all mice by day three of diet exposure. Total protein was reduced by only 9% and albumin was unchanged. Female mice of both strains had significantly lower alpha 1-antitrypsin levels than male mice prior to and after diet exposure. This was associated with significantly greater mortality in both female strains. Interstrain comparisons showed a significantly higher mortality in the C57BL-6 females (100%) compared to the ICR females (58%); this corresponded to significantly lower alpha 1-antitrypsin levels in C57BL-6 females. Regardless of sex or strain, alpha 1-antitrypsin levels prior to and after diet exposure were significantly higher in mice surviving than in mice dying. We conclude that circulating alpha 1-antitrypsin is a predictor of mortality from diet-induced pancreatitis.
在小鼠中，含乙硫氨酸且胆碱缺乏的饮食可诱发实验性胰腺炎。我们使用两种品系的小鼠（ICR和C57BL - 6）研究了循环α1 - 抗胰蛋白酶在此模型中的作用。在饮食暴露的第三天，所有小鼠的循环α1 - 抗胰蛋白酶水平降低了50%。总蛋白仅降低了9%，白蛋白未发生变化。在饮食暴露前后，两个品系的雌性小鼠的α1 - 抗胰蛋白酶水平均显著低于雄性小鼠。这与两个雌性品系中显著更高的死亡率相关。品系间比较显示，C57BL - 6雌性小鼠（100%）的死亡率显著高于ICR雌性小鼠（58%）；这与C57BL - 6雌性小鼠中显著更低的α1 - 抗胰蛋白酶水平相对应。无论性别或品系如何，饮食暴露前后存活小鼠的α1 - 抗胰蛋白酶水平均显著高于死亡小鼠。我们得出结论，循环α1 - 抗胰蛋白酶是饮食诱导的胰腺炎死亡率的一个预测指标。
