Formosa Cécile, Schiavone Marion, Boisrame Anita, Richard Mathias L, Duval Raphaël E, Dague Etienne
CNRS, LAAS, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31400 Toulouse, France; CNRS, UMR 7565, SRSMC, Vandœuvre-lès-Nancy, France; Université de Lorraine, UMR 7565, Faculté de Pharmacie, Nancy, France.
CNRS, LAAS, F-31400 Toulouse, France; Université de Toulouse, LAAS, F-31400 Toulouse, France.
Nanomedicine. 2015 Jan;11(1):57-65. doi: 10.1016/j.nano.2014.07.008. Epub 2014 Aug 5.
Candida albicans is an opportunistic pathogen. It adheres to mammalian cells through a variety of adhesins that interact with host ligands. The spatial organization of these adhesins on the cellular interface is however poorly understood, mainly because of the lack of an instrument able to track single molecules on single cells. In this context, the atomic force microscope (AFM) makes it possible to analyze the force signature of single proteins on single cells. The present study is dedicated to the mapping of the adhesive properties of C. albicans cells. We observed that the adhesins at the cell surface were organized in nanodomains composed of free or aggregated mannoproteins. This was demonstrated by the use of functionalized AFM tips and synthetic amyloid forming/disrupting peptides. This direct visualization of amyloids nanodomains will help in understanding the virulence factors of C. albicans.
白色念珠菌是一种机会致病菌。它通过多种与宿主配体相互作用的黏附素黏附于哺乳动物细胞。然而，这些黏附素在细胞界面上的空间组织情况却知之甚少，主要是因为缺乏能够在单细胞上追踪单个分子的仪器。在这种情况下，原子力显微镜（AFM）使得分析单细胞上单个蛋白质的力特征成为可能。本研究致力于绘制白色念珠菌细胞的黏附特性图谱。我们观察到细胞表面的黏附素以由游离或聚集的甘露糖蛋白组成的纳米结构域形式存在。这通过使用功能化的AFM探针和合成的淀粉样蛋白形成/破坏肽得以证明。淀粉样蛋白纳米结构域的这种直接可视化将有助于理解白色念珠菌的毒力因子。
