增强联系：淀粉样蛋白在酵母中形成黏附纳米结构域。
Strengthening relationships: amyloids create adhesion nanodomains in yeasts.
机构信息
Department of Biology, Brooklyn College of the City University of New York, New York, NY, USA.
出版信息
Trends Microbiol. 2012 Feb;20(2):59-65. doi: 10.1016/j.tim.2011.10.002. Epub 2011 Nov 16.
Abstract
Budding yeasts adhere to biotic or abiotic surfaces and aggregate to form biofilms, using wall-anchored glycoprotein adhesins. The process is paradoxical: adhesins often show weak binding to specific ligands, yet mediate remarkably strong adherence. Single-molecule atomic force microscopy (AFM), genomics, biochemistry and cell biology have recently explained the puzzle, with Candida albicans Als adhesins as the paradigm. The strength of adhesion results partly from force-activated amyloid-like clustering of hundreds of adhesin molecules to form arrays of ordered multimeric binding sites. The various protein domains of eukaryotic adhesins cooperate to facilitate this fascinating new mechanism of activation.
摘要
出芽酵母附着在生物或非生物表面，并通过壁锚定糖蛋白黏附素聚集形成生物膜。这个过程是矛盾的：黏附素通常与特定配体的结合较弱，但介导的黏附作用却非常强。单细胞原子力显微镜（AFM）、基因组学、生物化学和细胞生物学最近解释了这个难题，以白色念珠菌 Als 黏附素为例。黏附的强度部分来自于数百个黏附素分子在力的作用下激活形成有序多聚体结合位点的淀粉样聚集。真核黏附素的各种蛋白结构域协同作用，促进了这种迷人的新激活机制。
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1
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Trends Microbiol. 2012 Feb;20(2):66-73. doi: 10.1016/j.tim.2011.11.005. Epub 2011 Dec 23.
2
Structural basis for the broad specificity to host-cell ligands by the pathogenic fungus Candida albicans.致病真菌白念珠菌对宿主细胞配体具有广泛特异性的结构基础。
Proc Natl Acad Sci U S A. 2011 Sep 20;108(38):15775-9. doi: 10.1073/pnas.1103496108. Epub 2011 Sep 6.
3
Amyloid of the Candida albicans Ure2p prion domain is infectious and has an in-register parallel β-sheet structure.白色念珠菌 Ure2p 朊病毒结构域的淀粉样蛋白具有感染性，并且具有规则平行的β-折叠结构。
Biochemistry. 2011 Jul 12;50(27):5971-8. doi: 10.1021/bi200142x. Epub 2011 Jun 15.
4
The N-terminal part of Als1 protein from Candida albicans specifically binds fucose-containing glycans.白念珠菌 Als1 蛋白的 N 端部分特异性结合含有岩藻糖的聚糖。
Mol Microbiol. 2011 Jun;80(6):1667-79. doi: 10.1111/j.1365-2958.2011.07676.x. Epub 2011 May 17.
5
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J Biol Chem. 2011 May 13;286(19):16533-40. doi: 10.1074/jbc.R111.227108. Epub 2011 Mar 25.
6
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PLoS One. 2011 Mar 8;6(3):e17632. doi: 10.1371/journal.pone.0017632.
7
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8
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Semin Cell Dev Biol. 2011 Jul;22(5):460-8. doi: 10.1016/j.semcdb.2011.02.019. Epub 2011 Feb 18.
9
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Nat Rev Microbiol. 2011 Feb;9(2):109-18. doi: 10.1038/nrmicro2475. Epub 2010 Dec 29.
10
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