Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University , 152 Luoyu Road, Wuhan 430079, P. R. China.
Anal Chem. 2014 Sep 2;86(17):8835-41. doi: 10.1021/ac502227p. Epub 2014 Aug 15.
The development of probes for rapid, selective, and sensitive detection of the highly toxic thiophenols is of great importance in both environmental and biological science. Despite the appealing advantages of near-infrared (NIR) fluorescent detection, no NIR fluorescent probes have been reported for thiophenols to date. Using the chemical properties of thiophenols that are able to cleave sulfonamide selectively and efficiently under mild conditions, we herein report a dicyanomethylene-benzopyran (DCMB)-based NIR fluorescent probe for thiophenols. This probe features remarkable large Stokes shift and shows a rapid, highly selective, and sensitive detection process for thiophenols with significant NIR fluorescent turn-on responses. The potential applications of this new NIR fluorescent probe were demonstrated by the quantitative detection of thiophenol in real water samples and by fluorescent imaging of thiophenol in living cells.
开发用于快速、选择性和灵敏检测高毒性噻吩酚的探针在环境和生物科学中具有重要意义。尽管近红外（NIR）荧光检测具有吸引人的优势，但迄今为止尚未有报道用于检测噻吩酚的 NIR 荧光探针。利用噻吩酚能够在温和条件下选择性和有效地切断磺酰胺键的化学性质，我们在此报告了一种基于二氰基乙烯基-苯并吡喃（DCMB）的 NIR 荧光探针用于检测噻吩酚。该探针具有显著的大斯托克斯位移，并对噻吩酚具有快速、高选择性和灵敏的检测过程，具有显著的 NIR 荧光开启响应。通过对实际水样中噻吩酚的定量检测以及对活细胞中噻吩酚的荧光成像，证明了这种新的 NIR 荧光探针的潜在应用。
