Shanghai Key Laboratory of Functional Materials Chemistry, Key Laboratory for Advanced Materials and Institute of Fine Chemicals, School of Chemistry and Molecular Engineering, East China University of Science & Technology, Shanghai 200237, PR China.
National Food Quality Supervision and Inspection Center (Shanghai) / Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China.
Talanta. 2018 Aug 1;185:359-364. doi: 10.1016/j.talanta.2018.03.062. Epub 2018 Mar 22.
Developing fluorescent probes for specific detection of extremely toxic thiophenols is pretty significant in the field of environment, chemistry and biology. We report herein a turn-on red fluorescent xanthene-based probe (RD-Probe) for detecting thiophenol with high selectivity over other analytes including aliphatic thiols. The probe could play the part of a "naked-eye"colorimetric indicator toward thiophenol. Moreover, the relative fluorescence intensity at 653 nm displayed good linearity with the concentration of thiophenol ranging from 0 to 6 μM, and the limit of detection for thiophenol could be as low as 15 nM. Furthermore, the practicability of RD-Probe has been successfully proved through the quantitative thiophenol detection in real water samples and fluorescence bioimaging of thiophenol in HeLa cells.
开发用于特异性检测极毒巯基苯的荧光探针在环境、化学和生物学领域具有重要意义。我们在此报告了一种基于呫吨的荧光探针（RD-Probe），它可以高选择性地检测巯基苯，而对其他分析物（包括脂肪族硫醇）则没有选择性。该探针可以作为巯基苯的“肉眼”比色指示剂。此外，在 653nm 处的相对荧光强度与巯基苯的浓度在 0 到 6μM 范围内呈良好的线性关系，巯基苯的检测限可低至 15nM。此外，RD-Probe 的实用性已通过实际水样中巯基苯的定量检测和 HeLa 细胞中巯基苯的荧光生物成像得到了成功验证。
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