Semeshin V F, Zhimulëv I F
Tsitologiia. 1989 Jun;31(6):728-31.
The following rules of the polytene chromosome mapping at the submicroscopical level are proposed: 1) using anhydrous mixtures of alcohol with acetic acid for fixation of salivary glands which permits to avoid artifacts in the structure of large bands; 2) using sections 120-150 nm thick which improves the revealing of faint bands; 3) including in the analysis larvae being at different stages, since different bands display puffing at different regions; 4) reproducibility of banding pattern in serial sections of some chromosomes. The use of these rules permits to interpret more strictly the observed pictures of the polytene chromosome banding pattern and to avoid possible mistakes of the mapping.
1）使用酒精与乙酸的无水混合物固定唾液腺，这有助于避免大条带结构中出现假象；2）使用120 - 150纳米厚的切片，这能改善微弱条带的显示效果；3）在分析中纳入处于不同阶段的幼虫，因为不同的条带在不同区域呈现胀泡现象；4）某些染色体连续切片中带型的可重复性。运用这些规则能够更严格地解释所观察到的多线染色体带型图谱，并避免图谱绘制中可能出现的错误。
