Barskiĭ V E, Anan'ev E V
Mol Biol (Mosk). 1985 Jan-Feb;19(1):295-301.
Electron microscopic study of total polytene chromosome preparations makes it possible to reveal elementary structures of polytene chromosomes. Elementary chromosomes are detected which contain chromomeric and interchromomeric regions. Transcription is seen in puffs and in many bands. Chromatin spreading shows that both chromomeric and interchromomeric regions of elementary chromosomes consist of nucleosomes. A ribbon-like model of polytene chromosome structure is suggested and discussed. In the ribbon-like chromosome each elementary chromosome gels conjugated only with the two adjacent sister chromosomes. Conjugation occurs only in the chromomeric regions, interchromomeric regions do not conjugate. Both chromomeric and interchromomeric regions are duplicated during polytenization which ensure proportional replication of all the bands and interbands in the course of polytenization.
对整个多线染色体标本进行电子显微镜研究，能够揭示多线染色体的基本结构。可检测到含有染色粒和染色粒间区域的基本染色体。在胀泡和许多条带中可见转录现象。染色质铺展显示，基本染色体的染色粒和染色粒间区域均由核小体组成。提出并讨论了多线染色体结构的带状模型。在带状染色体中，每个基本染色体仅与相邻的两条姐妹染色体结合。结合仅发生在染色粒区域，染色粒间区域不结合。在多线化过程中，染色粒和染色粒间区域都会复制，这确保了在多线化过程中所有条带和间带的比例复制。
