Zoldan Katharina, Moellmer Theresa, Schneider Josephine, Fueldner Christiane, Knauer Jens, Lehmann Jörg
Department of Cell Engineering, Fraunhofer Institute for Cell Therapy and Immunology, Perlickstr. 1, 04103 Leipzig, Germany.
Saxon State Office for Environment, Agriculture and Geology, Am Park 3, 04886 Köllitsch, Germany.
Dev Comp Immunol. 2014 Dec;47(2):254-63. doi: 10.1016/j.dci.2014.08.002. Epub 2014 Aug 11.
Polymorph-nuclear neutrophils (PMN) in cattle exhibit unique features when compared to human or murine PMN and are of particular interest concerning the risk of post-partum mammary gland or extra-mammary infections related to the periparturient suppression of neutrophil functions. Former studies could show that effects of IL-2 on innate immune cells such as PMN were mediated by the interleukin-2 receptor (IL-2R) β and γ chains. In the current study we could detect IL-2Rα (CD25) expression on bovine PMN using flow-cytometric analysis. CD25 was detected on granulocytes from post-partum and early lactating cows with different inflammatory conditions. The expression of CD25 on PMN in blood and raw milk increased with disease severity. Our results suggest CD25 expression on PMN as a potential biomarker for acute infections in cattle. Furthermore, our data provide a basis to better understanding of the periparturient functional suppressions of PMN that might reveal new molecular targets for therapy or prevention of disease.
