Chen Wen-Ming, Chen Jhen-Ci, Sheu Shih-Yi
Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142 Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Int J Syst Evol Microbiol. 2014 Nov;64(Pt 11):3701-3708. doi: 10.1099/ijs.0.065524-0. Epub 2014 Aug 8.
A bacterial strain, designated Jyi-05(T), was isolated from a flooded rice field and characterized using a polyphasic taxonomic approach. Cells of strain Jyi-05(T) were Gram-stain-negative, strictly aerobic, bright-yellow-pigmented rods that were motile by gliding. Growth occurred at 10-30 °C (optimum 30 °C), at pH 6.5-7.5 (optimum pH 7.0) and with 0-2% (w/v) NaCl (optimum 1%, w/v, NaCl). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain Jyi-05(T) belonged to the genus Flavobacterium and was most closely related to Flavobacterium flevense DSM 1076(T) with sequence similarity of 97.6%. Strain Jyi-05(T) contained iso-C(15:0) (16.6%), anteiso-C(15:0) (10.8%), summed feature 3 (C(16:1)ω7c and/or C(16:1)ω6c; 10.3%), iso-C((17:0) 3-OH (9.2%), C(16:0) (7.3%) and iso-C(15:0) 3-OH (7.2%) as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine and several uncharacterized aminophospholipids and phospholipids. The major polyamine was homospermidine. The genomic DNA G+C content of strain Jyi-05(T) was 34.2 mol%. The DNA-DNA relatedness of strain Jyi-05(T) with respect to recognized species of the genus Flavobacterium was less than 70%. On the basis of the phylogenetic inference and phenotypic data, strain Jyi-05(T) should be classified as a novel species, for which the name Flavobacterium oryzae sp. nov. is proposed. The type strain is Jyi-05(T) ( = BCRC 80522(T) =LMG 27292(T) =KCTC 32240(T)). Emended descriptions of Flavobacterium flevense, Flavobacterium yonginense and Flavobacterium myungsuense are also presented.
从淹水的稻田中分离出一株细菌，命名为Jyi-05(T)，并采用多相分类方法对其进行了表征。Jyi-05(T)菌株的细胞革兰氏染色阴性，严格需氧，呈亮黄色，为可滑行运动的杆菌。该菌株在10-30℃（最适温度30℃）、pH 6.5-7.5（最适pH 7.0）和0-2%（w/v）NaCl（最适1%，w/v，NaCl）条件下生长。基于16S rRNA基因序列的系统发育分析表明，Jyi-05(T)菌株属于黄杆菌属，与弗勒维黄杆菌DSM 1076(T)关系最为密切，序列相似性为97.6%。Jyi-05(T)菌株的主要脂肪酸包括异-C(15:0)（16.6%）、anteiso-C(15:0)（10.8%）、总和特征3（C(16:1)ω7c和/或C(16:1)ω6c；10.3%）、异-C(17:0) 3-OH（9.2%）、C(16:0)（7.3%）和异-C(15:0) 3-OH（7. (2%)。主要的类异戊二烯醌为MK-6。极性脂质谱由磷脂酰乙醇胺和几种未鉴定的氨基磷脂及磷脂组成。主要的多胺为高亚精胺。Jyi-05(T)菌株的基因组DNA G+C含量为34.2 mol%。Jyi-05(T)菌株与黄杆菌属已确认物种的DNA-DNA相关性低于70%。基于系统发育推断和表型数据，Jyi-05(T)菌株应被分类为一个新物种，为此提出黄杆菌水稻新种的名称。模式菌株为Jyi-05(T)（=BCRC 80522(T)=LMG 27292(T)=KCTC 32240(T)）。同时还给出了弗勒维黄杆菌、龙仁黄杆菌和明秀黄杆菌的修订描述。
