Sheu Shih-Yi, Su Ching-Ling, Kwon Soon-Wo, Chen Wen-Ming
Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Dec;67(12):5179-5186. doi: 10.1099/ijsem.0.002443. Epub 2017 Oct 26.
Strain KYPY10, isolated from a water sample taken from the Funglin Stream in Taiwan, was characterized using a polyphasic taxonomic approach. Cells of strain KYPY10 were Gram-stain-negative, strictly aerobic, motile by gliding, rod-shaped surrounded by a thick capsule, and formed light-yellow colonies. Growth occurred at 20-30 °C (optimum, 25 °C), at pH 6-7 (optimum, pH 6) and with 0-0.2 % NaCl (optimum, 0 % w/v). Phylogenetic analyses, based on 16S rRNA gene sequences, showed that strain KYPY10 belonged to the genus Flavobacterium and was most closely related to Flavobacterium brevivitae TTM-43 (98.4 % sequence similarity) and Flavobacterium vireti THG-SM1 (98.0 %). Strain KYPY10 contained iso-C15 : 0, iso-C15 : 1 G, summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0), iso-C17 : 0 3-OH, iso-C15 : 0 3-OH and iso-C16 : 0 as the predominant fatty acids. The major isoprenoid quinone was MK-6. The polar lipid profile consisted of phosphatidylethanolamine, four uncharacterized aminophospholipids, five uncharacterized phospholipids and one uncharacterized lipid. The major polyamines were homospermidine and putrescine. The genomic DNA G+C content of strain KYPY10 was 41.0 mol%. The DNA-DNA relatedness of strain KYPY10 with respect to recognized species of the genus Flavobacterium was less than 70 %. On the basis of the phylogenetic inference and phenotypic data, strain KYPY10 was recognized as a representative of a novel species within the genus Flavobacterium. The name Flavobacterium amniphilum sp. nov. is proposed, with strain KYPY10 (=BCRC 81006=LMG 29727=KCTC 52443) as the type strain.
从台湾枫林溪采集的水样中分离出的菌株KYPY10，采用多相分类法进行了特征描述。菌株KYPY10的细胞革兰氏染色阴性，严格需氧，通过滑行运动，杆状，周围有一层厚荚膜，形成浅黄色菌落。在20-30°C（最适温度为25°C）、pH值6-7（最适pH值为6）和0-0.2%氯化钠（最适浓度为0% w/v）条件下生长。基于16S rRNA基因序列的系统发育分析表明，菌株KYPY10属于黄杆菌属，与短黄杆菌TTM-43（序列相似性为98.4%）和维氏黄杆菌THG-SM1（序列相似性为98.0%）关系最为密切。菌株KYPY10以异-C15:0、异-C15:1 G、总和特征9（异-C17:1ω9c和/或10-甲基-C16:0）、异-C17:0 3-OH、异-C15:0 3-OH和异-C16:0作为主要脂肪酸。主要类异戊二烯醌为MK-6。极性脂质谱由磷脂酰乙醇胺、四种未鉴定的氨基磷脂、五种未鉴定的磷脂和一种未鉴定的脂质组成。主要多胺为高亚精胺和腐胺。菌株KYPY10的基因组DNA G+C含量为41.0 mol%。菌株KYPY10与黄杆菌属已确认物种的DNA-DNA相关性小于70%。基于系统发育推断和表型数据，菌株KYPY10被确认为黄杆菌属内一个新物种的代表。提议将其命名为嗜水黄杆菌新种，以菌株KYPY10（=BCRC 81006=LMG 29727=KCTC 52443）作为模式菌株。
