Tsuchiya Yugo, Pham Uyen, Gout Ivan
*Institute of Structural and Molecular Biology, University College London, Gower Street, London WC1E 6BT, U.K.
Biochem Soc Trans. 2014 Aug;42(4):1107-11. doi: 10.1042/BST20140123.
CoA (coenzyme A) is a ubiquitous and essential cofactor that acts as an acyl group carrier in biochemical reactions. Apart from participating in numerous metabolic pathways as substrates and intermediates, CoA and a number of its thioester derivatives, such as acetyl-CoA, can also directly regulate the activity of proteins by allosteric mechanisms and by affecting protein acetylation reactions. Cellular levels of CoA and CoA thioesters change under various physiological and pathological conditions. Defective CoA biosynthesis is implicated in NBIA (neurodegeneration with brain iron accumulation). However, the exact role of CoA in the pathogenesis of NBIA is not well understood. Accurate and reliable assays for measuring CoA species in biological samples are essential for studying the roles of CoA and CoA derivatives in health and disease. The present mini-review discusses methods that are commonly used to measure CoA species in biological samples.
辅酶A（CoA）是一种普遍存在且必不可少的辅助因子，在生化反应中作为酰基载体发挥作用。除了作为底物和中间体参与众多代谢途径外，辅酶A及其一些硫酯衍生物，如乙酰辅酶A，还可通过变构机制和影响蛋白质乙酰化反应直接调节蛋白质的活性。在各种生理和病理条件下，辅酶A和辅酶A硫酯的细胞水平会发生变化。辅酶A生物合成缺陷与神经退行性脑铁沉积症（NBIA）有关。然而，辅酶A在NBIA发病机制中的确切作用尚不清楚。准确可靠地测定生物样品中辅酶A种类的分析方法对于研究辅酶A及其衍生物在健康和疾病中的作用至关重要。本综述讨论了常用于测定生物样品中辅酶A种类 的方法。
