Department of Applied Life Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo 183-8509, Japan.
Biochem Biophys Res Commun. 2010 Nov 5;402(1):158-62. doi: 10.1016/j.bbrc.2010.10.009. Epub 2010 Oct 8.
Levels of three coenzyme A (CoA) molecular species, i.e., nonesterified CoA (CoASH), acetyl-CoA, and malonyl-CoA, in fasted and fed rat tissues were analyzed by the acyl-CoA cycling method which makes detection possible at the pmol level. Malonyl-CoA in brain tissues readily increased with feeding, and inversely, acetyl-CoA decreased. This phenomenon occurred in the cerebral cortex, hippocampus, cerebellum, and medulla oblongata, as well as in the hypothalamus which controls energy balance by monitoring malonyl-CoA. In the non-brain tissues, the sizes of the acetyl-CoA, malonyl-CoA, and CoASH pools depended on the tissues. The total CoA pools consisting of the above three CoA species in the liver, heart, and brown adipose tissue were larger and those of the perirenal, epididymal, and ovarian adipose tissues were much smaller, compared with those of other tissues including brain tissues. In addition, the response of each CoA pool to feeding was not uniform, suggesting that the tissue-specific metabolism individually functions in the non-brain tissues. Thus, a comprehensive analysis of thirteen types of rat tissue revealed that CoA pools have different sizes and showed a different response to fasting and feeding depending on the tissue.
采用酰基辅酶 A 循环法分析了禁食和进食状态下大鼠组织中三种辅酶 A（CoA）分子的水平，即非酯化 CoA（CoASH）、乙酰 CoA 和丙二酰 CoA，该方法可在皮摩尔水平进行检测。脑组织中的丙二酰 CoA 随着进食而迅速增加，相反，乙酰 CoA 则减少。这种现象发生在大脑皮层、海马体、小脑和延髓，以及控制能量平衡的下丘脑，通过监测丙二酰 CoA 来控制能量平衡。在非脑组织中，乙酰 CoA、丙二酰 CoA 和 CoASH 池的大小取决于组织。肝脏、心脏和棕色脂肪组织中由上述三种 CoA 组成的总 CoA 池较大，而肾周、附睾和卵巢脂肪组织的总 CoA 池则小得多，与包括脑组织在内的其他组织相比。此外，每种 CoA 池对进食的反应并不一致，这表明非脑组织中的个体组织具有特定的代谢功能。因此，对 13 种类型的大鼠组织进行全面分析表明，CoA 池的大小不同，对禁食和进食的反应也因组织而异。
