Li Qiang, Xu Fen, Liang Feng-Xia, Chen Rui, Wu Song, Liu Jian-Min, Yang Liu, Min Fei, Ye Jin-Tian
Zhongguo Zhen Jiu. 2014 Jun;34(6):578-82.
To explore the mechanism of electroacupuncture on improving insulin resistance of rat from aspects of morphology and function of mitochondrial in quadriceps femoris.
Forty-eight 8-week Wistar rats (female and male in half) were randomly divided into a normal group (16 rats, group A), a model control group (16 rats, group B), a model plus electroacupuncture (EA) group (8 rats, group C) and a model plus sham acupoint EA group (8 rats, group D). Group A was given with basic diet while high-fat diet was applied in the group B, group C and group D for 8 weeks to establish model of insulin resistance. After the model establishment, "Guanyuan" (CV 4), "Zhongwan" (CV 12), "Zusanli" (ST 36) and "Fenglong" (ST 30) were selected according to acupoint combination of manifestation-root in the group C, while four points in non-meridian area where 1 to 2 mm next to the acupoints used in group C were selected in the group D. The treatment was given 15 min per time with 1 mA of intensity and 2 Hz in frequency, 5 times per week for totally 8 weeks. The transmission electron microscope was adopted to observe mitochondria structure, and chemical colorimetry was used to test the activity of adenosine triphosphate (ATP) synthase and phosphomolybdic acid colorimetry was applied to measure the content of ATP.
After the treatment, the body mass was (401.63 +/- 109.81) g in the group B, which was significantly higher than (305.88 +/- 62.72) g in the group A (P < 0.05); morphological structure of mitochondrion was damaged, showing swelling and deformation; the activity of ATP synthase was decreased (P < 0.05) and the content of ATP in tissue of quadriceps femoris was also obviously lowered (P < 0.05). The body mass was (294.13 +/- 53.78) g in the group C, which was significantly lower than that in the group B (P < 0.05); the damaged mitochondrion was restored and merged among each other; the activity of ATP synthase was increased (P < 0.05); the content of ATP in tissue of quadriceps femoris was obviously lifted (P < 0.05). The results in group D were not different from those in group B.
The electroacupuncture with manifestation-root acupoint combination could improve the recovery of damaged structure of mitochondrion and promote the merge among each other, which could enhance oxidizing capacity, lower body mass and improve synthetic rate of ATP.
从股四头肌线粒体形态和功能方面探讨电针对改善大鼠胰岛素抵抗的机制。
将48只8周龄Wistar大鼠（雌雄各半）随机分为正常组（16只，A组）、模型对照组（16只，B组）、模型加电针（EA）组（8只，C组）和模型加假穴电针组（8只，D组）。A组给予基础饲料，B、C、D组给予高脂饲料8周以建立胰岛素抵抗模型。造模成功后，C组根据标本根结配穴法选取“关元”（CV4）、“中脘”（CV12）、“足三里”（ST36）、“丰隆”（ST30）；D组选取距C组穴位旁开1～2mm的非经非穴区四点。每次治疗15分钟，强度1mA，频率2Hz，每周5次，共8周。采用透射电子显微镜观察线粒体结构，化学比色法检测三磷酸腺苷（ATP）合酶活性，磷钼酸比色法测定ATP含量。
治疗后，B组体质量为（401.63±109.81）g，明显高于A组的（305.88±62.72）g（P＜0.05）；线粒体形态结构受损，出现肿胀、变形；ATP合酶活性降低（P＜0.05），股四头肌组织中ATP含量也明显降低（P＜0.05）。C组体质量为（294.13±53.78）g，明显低于B组（P＜0.05）；受损线粒体恢复且相互融合；ATP合酶活性升高（P＜0.05）；股四头肌组织中ATP含量明显升高（P＜0.05）。D组结果与B组无差异。
标本根结配穴电针可改善受损线粒体结构的恢复并促进其相互融合，增强氧化能力，降低体质量，提高ATP合成率。
