Brosnan C F, Litwak M S, Schroeder C E, Selmaj K, Raine C S, Arezzo J C
Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461.
J Neuroimmunol. 1989 Dec;25(2-3):227-39. doi: 10.1016/0165-5728(89)90141-0.
Epidural visual evoked potentials (VEP) were used to study the role of cytokines in the induction of pathophysiologic changes associated with inflammation in the central nervous system (CNS) of the rabbit. In normal rabbits, intraocular injection of human recombinant interferon-gamma (IFN-gamma) and tumor necrosis factor (TNF) increased the peak latency of the cortical VEP by more than 2 ms within 3 h of injection; equal volume injections of control substances had no effect. Alterations in conduction induced by IFN-gamma and TNF reversed within 24 h and could be reinduced by reinjection. Intraocular injection of recombinant human interleukin-1 beta (IL-1) induced a more progressive delay in conduction that peaked 24 h after intraocular challenge and reversed over the ensuing 48 h. Pathologic examination of the tissues indicated that the primary effect of these cytokines is on the vasculature and induces changes associated with inflammation. The results suggest that the acute reversible effects of cytokines on CNS function are associated with vascular events; further they support the sensitivity of the 'rabbit eye model' for studies on the pathophysiologic effect of inflammatory mediators on the CNS in vivo.
采用硬膜外视觉诱发电位（VEP）来研究细胞因子在兔中枢神经系统（CNS）炎症相关病理生理变化诱导过程中的作用。在正常兔中，眼内注射人重组干扰素-γ（IFN-γ）和肿瘤坏死因子（TNF）在注射后3小时内使皮质VEP的峰潜伏期增加超过2毫秒；注射等体积的对照物质则无影响。IFN-γ和TNF诱导的传导改变在24小时内逆转，且再次注射可再次诱导。眼内注射重组人白细胞介素-1β（IL-1）导致传导延迟更为渐进，在眼内激发后24小时达到峰值，并在随后的48小时内逆转。组织病理学检查表明，这些细胞因子的主要作用是对脉管系统，并诱导与炎症相关的变化。结果表明，细胞因子对中枢神经系统功能的急性可逆作用与血管事件有关；此外，它们支持“兔眼模型”对体内炎症介质对中枢神经系统病理生理作用研究的敏感性。
