Gerhard Rene, Schmitt Fernando C
Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, and Department of Pathology, University Health Network, Toronto, Ont., Canada.
Acta Cytol. 2014;58(6):533-42. doi: 10.1159/000362805. Epub 2014 Aug 9.
Fine-needle aspiration (FNA) is a safe and cost-effective technique for the diagnosis of breast lesions, especially when correlated with clinical and imaging studies. However, the success of breast FNA is highly dependent on the adequate preparation of cytological conventional smears (CS). The liquid-based cytology (LBC) technique consists of an automated method for preparing thin-layer cytological samples from cell suspensions collected in alcohol-based preservative. LBC is designed to improve CS by avoiding limiting factors such as obscuring material, air-drying and smearing artifacts.
We performed a review of the published literature about LBC applied to breast FNA.
LBC preparations of breast aspirates demonstrated better cellular preservation, less cell overlapping and elimination of blood and excessive inflammation compared to CS. Conversely, alterations in architecture and cell morphology as well as loss of myoepithelial cells and stromal elements have been described in LBC specimens, requiring training before applying this technique for diagnosis. Studies have shown a similar accuracy between LBC and CS for the diagnosis of breast lesions. LBC also permits the use of residual material for ancillary tests, which is an important advantage compared to CS.
LBC can be safely applied to breast FNA, showing a similar diagnostic accuracy to CS.
细针穿刺抽吸术（FNA）是诊断乳腺病变的一种安全且经济有效的技术，尤其是与临床及影像学检查相结合时。然而，乳腺FNA的成功高度依赖于细胞常规涂片（CS）的充分制备。液基细胞学（LBC）技术是一种从收集于酒精基防腐剂中的细胞悬液制备薄层细胞学样本的自动化方法。LBC旨在通过避免诸如遮盖物、空气干燥和涂片假象等限制因素来改进CS。
我们对已发表的关于应用于乳腺FNA的LBC的文献进行了综述。
与CS相比，乳腺抽吸物的LBC制片显示出更好的细胞保存、更少的细胞重叠以及血液和过度炎症的消除。相反，LBC标本中已描述了结构和细胞形态的改变以及肌上皮细胞和间质成分的丢失，在应用该技术进行诊断之前需要培训。研究表明，LBC和CS在乳腺病变诊断方面具有相似的准确性。LBC还允许将剩余材料用于辅助检查，这是与CS相比的一个重要优势。
LBC可安全应用于乳腺FNA，显示出与CS相似的诊断准确性。
