Kumar Madhu, Katiyar Shweta, Sagar Mala, Kumari Malti, Goel Madhu Mati
Department of Pathology, King George's Medical University, Lucknow, Uttar Pradesh, India.
Indian J Pathol Microbiol. 2018 Jan-Mar;61(1):45-49. doi: 10.4103/IJPM.IJPM_729_16.
Liquid-based cytology (LBC) is a method of retrieving and processing of cytological material for the assessment of both gynecological and nongynecological cases introduced in 1996. Mostly conventional smears (CS) are prepared in Indian scenario; however, due to increasing popularity of LBC in nongynecology specimens, LBC is also used in few centers for diagnosing salivary gland neoplasm.
The aim of this study is to compare CS and LBC in fine-needle aspiration cytology (FNAC) of the salivary gland neoplasms in terms of cytomorphological details, adequacy, ease of interpretation, pitfalls, and diagnostic efficiency.
We conducted a prospective, observational, comparative study which included 64 salivary gland neoplasms. Both CS and LBC (SurePath) were prepared as per standard protocols and examined.
In our study, specificity and positive predictive value of both the techniques (conventional and LBC) were found to be 100%. Sensitivity of both the techniques was found to be similar (66.7%). Negative predictive value and diagnostic accuracy of conventional technique were found to be almost similar to that of LBC technique (97.6% vs. 97.2% and 97.7% vs. 97.4%). In terms of adequacy and cellularity, CSs were better than LBC. Ease of interpretation was better with CSs due to abundant chondromyxoid stroma, an important clue in the diagnosis of pleomorphic adenoma. Nuclear details and background were better in LBC as compared to CS. Hence, we conclude that in salivary gland FNAC, both CS and LBC have similar diagnostic efficiency. however, interpretation of conventional smears is easier than Liquid Based Cytology especially in cases of pleomorphic adenoma. However, some advantages of LBC in respect to conventional cytology, with better-preserved morphology obtained from LBC becomes furthermore obligatory.
液基细胞学（LBC）是一种用于获取和处理细胞学材料以评估妇科和非妇科病例的方法，于1996年引入。在印度的情况下，大多制备传统涂片（CS）；然而，由于LBC在非妇科标本中越来越受欢迎，LBC也在少数中心用于诊断唾液腺肿瘤。
本研究的目的是在唾液腺肿瘤的细针穿刺细胞学检查（FNAC）中，比较CS和LBC在细胞形态学细节、充分性、解读的难易程度、陷阱和诊断效率方面的差异。
我们进行了一项前瞻性、观察性、比较性研究，纳入了64例唾液腺肿瘤。按照标准方案制备CS和LBC（SurePath）并进行检查。
在我们的研究中，发现两种技术（传统技术和LBC）的特异性和阳性预测值均为100%。发现两种技术的敏感性相似（66.7%）。发现传统技术的阴性预测值和诊断准确性与LBC技术几乎相似（97.6%对97.2%以及97.7%对97.4%）。在充分性和细胞含量方面，CS优于LBC。由于存在丰富的软骨黏液样基质，这是诊断多形性腺瘤的重要线索，CS的解读更容易。与CS相比，LBC的核细节和背景更好。因此，我们得出结论，在唾液腺FNAC中，CS和LBC具有相似的诊断效率。然而，传统涂片的解读比液基细胞学更容易，尤其是在多形性腺瘤的病例中。然而，LBC相对于传统细胞学的一些优势，即从LBC获得的形态保存更好，变得更加必要。
