Jeronymo-Ceneviva Ana Beatriz, de Paula Aline Teodoro, Silva Luana Faria, Todorov Svetoslav Dimitrov, Franco Bernadette Dora G Mello, Penna Ana Lúcia B
Department of Food Engineering and Technology, UNESP - Sao Paulo State University, Rua Cristóvão Colombo, 2265, Jardim Nazareth, São José do Rio Preto, 15.0540-000, Brazil.
Probiotics Antimicrob Proteins. 2014 Dec;6(3-4):141-56. doi: 10.1007/s12602-014-9166-2.
This study evaluated the probiotic properties (stability at different pH values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of β-galactosidase production, evaluation of the presence of genes encoding MapA and Mub adhesion proteins and EF-Tu elongation factor, and the presence of genes encoding virulence factor) of four LAB strains (Lactobacillus casei SJRP35, Leuconostoc citreum SJRP44, Lactobacillus delbrueckii subsp. bulgaricus SJRP57 and Leuconostoc mesenteroides subsp. mesenteroides SJRP58) which produced antimicrobial substances (antimicrobial peptides). The strains survived the simulated GIT modeled in MRS broth, whole and skim milk. In addition, auto-aggregation and the cell surface hydrophobicity of all strains were high, and various degrees of co-aggregation were observed with indicator strains. All strains presented low resistance to several antibiotics and survived in the presence of commercial drugs. Only the strain SJRP44 did not produce the β-galactosidase enzyme. Moreover, the strain SJRP57 did not show the presence of any genes encoding virulence factors; however, the strain SJRP35 presented vancomycin resistance and adhesion of collagen genes, the strain SJRP44 harbored the ornithine decarboxylase gene and the strain SJRP58 generated positive results for aggregation substance and histidine decarboxylase genes. In conclusion, the strain SJRP57 was considered the best candidate as probiotic cultures for further in vivo studies and functional food products development.
本研究评估了四株产生抗菌物质（抗菌肽）的乳酸菌菌株（干酪乳杆菌SJRP35、柠檬明串珠菌SJRP44、德氏保加利亚乳杆菌亚种SJRP57和肠系膜明串珠菌亚种SJRP58）的益生菌特性（在不同pH值和胆盐浓度下的稳定性、自聚集和共聚集、在抗生素和市售药物存在下的存活率、β-半乳糖苷酶产生的研究、编码MapA和Mub粘附蛋白以及EF-Tu延伸因子的基因的存在情况，以及编码毒力因子的基因的存在情况）。这些菌株在MRS肉汤、全脂牛奶和脱脂牛奶中模拟的胃肠道环境中存活。此外，所有菌株的自聚集和细胞表面疏水性都很高，并且观察到与指示菌株有不同程度的共聚集。所有菌株对几种抗生素的抗性较低，并且在市售药物存在下存活。只有菌株SJRP44不产生β-半乳糖苷酶。此外，菌株SJRP57未显示任何编码毒力因子的基因；然而，菌株SJRP35表现出对万古霉素的抗性和胶原基因的粘附，菌株SJRP44含有鸟氨酸脱羧酶基因，菌株SJRP58的聚集物质和组氨酸脱羧酶基因检测呈阳性。总之，菌株SJRP57被认为是进一步进行体内研究和开发功能性食品的最佳益生菌培养候选菌株。
