UR 1268, INRA Biopolymères Interactions Assemblages - Equipe FIP, B.P. 71627, 44316 Nantes Cedex 03, France.
Anaerobe. 2013 Apr;20:42-9. doi: 10.1016/j.anaerobe.2013.01.003. Epub 2013 Jan 26.
The aims of this study were to characterize inhibitory activity spectra, some probiotic properties and safety of Lactobacillus curvatus A61 for its future application in production of fermented foods. The studied strain was isolated from traditional homemade cheese manufactured in Azerbaijan. The cell-free supernatant of culture of Lb. curvatus A61 inhibited the growth of tested LAB, as well as of Listeria monocytogenes and Bacillus cereus strains. The strain presented antifungal activity and inhibited the growth of Cladosporium and Fusarium ssp. during co-cultivation on agar media. PCR amplification with specific primers revealed the presence of curvacin A encoding gene in Lb. curvatus A61. Bacteriocin produced by the studied strain was heat stable and active in a broad pH range, and in the presence of Triton X-20, Triton X-80, Triton X-100, β-mercaptoethanol, Na-EDTA, SDS and NaCl. The mode of action of bacteriocin against selected indicator strains was found to be bacteriostatic. Lb. curvatus A61 was resistant to physiological concentrations of bile salts and showed high auto-aggregation ability, as well as co-aggregation ability with pathogenic L. monocytogenes strains. It was sensitive to chloramphenicol, penicillin, tetracycline, ciprofloxacin and vancomycin, but resistant to ampicillin and gentamicin.
本研究旨在描述乳杆菌 A61 的抑制活性谱、一些益生菌特性和安全性，以便将其未来应用于发酵食品的生产。该研究菌株是从阿塞拜疆传统自制奶酪中分离出来的。Lb. curvatus A61 的无细胞培养上清液抑制了测试的 LAB 以及李斯特菌和蜡状芽孢杆菌的生长。该菌株具有抗真菌活性，并在琼脂培养基上共培养时抑制了青霉属和镰刀菌属的生长。用特异性引物进行 PCR 扩增显示 Lb. curvatus A61 存在 curvacin A 编码基因。研究菌株产生的细菌素热稳定，在较宽的 pH 范围内和 Triton X-20、Triton X-80、Triton X-100、β-巯基乙醇、Na-EDTA、SDS 和 NaCl 存在下具有活性。发现细菌素对选定指示菌株的作用模式为抑菌作用。Lb. curvatus A61 对生理浓度的胆汁盐具有抗性，并表现出高的自聚集能力，以及与致病性李斯特菌菌株的共聚集能力。它对氯霉素、青霉素、四环素、环丙沙星和万古霉素敏感，但对氨苄西林和庆大霉素有抗性。
