Mendes R F M, Araujo Neto R B, Nascimento M P S B C, Lima P S C
Universidade Federal do Piauí, Campus Universitário Ministro Petrônio Portella, Teresina, PI, Brasil.
Embrapa Meio-Norte, Teresina, PI, Brasil.
Genet Mol Res. 2014 Aug 1;13(3):5832-9. doi: 10.4238/2014.August.1.1.
Among members of the Fabaceae family, native to the Brazilian Caatinga, the species Poincianella pyramidalis and P. bracteosa exhibit particular potential as forage for cattle, sheep and goats. With the aim of establishing genetic relationships within Poincianella, random amplified polymorphic DNA analysis was performed on eight accessions of P. pyramidalis and two accessions of P. bracteosa, originating from the semiarid zone of the state of Piauí, northeastern Brazil, and present in the germplasm bank of Embrapa Meio Norte (Teresina, Piauí, Brazil). Amplification reactions using 11 selected arbitrary sequence primers generated 167 fragments with an overall polymorphism of 70.38%. Five monomorphic loci were generated exclusively in P. pyramidalis accessions, while three unique monomorphic loci were associated with P. bracteosa, and these represented potential species-specific markers. The similarity coefficients between Poincianella accessions were low (mean value 0.59) but with a wide variation (range 0.443 to 0.748). The similarity matrix and the dendrogram constructed using the unweighted pair group method allowed the separation of Poincianella accessions into two major clusters represented by the two distinct species, while the accessions of P. pyramidalis could be separated further into three subgroups. The high level of genetic diversity detected in the genus Poincianella could be used in future breeding programs to produce enhanced cultivars, although the variability could be better exploited if more specimens were collected from other locations within the semiarid region of northeastern Brazil.
在豆科植物中，原产于巴西卡廷加的Poincianella pyramidalis和P. bracteosa这两个物种作为牛、羊和山羊的饲料具有特别的潜力。为了确定Poincianella属内的遗传关系，对来自巴西东北部皮奥伊州半干旱地区、保存在巴西农牧业研究公司东北区域研究中心（位于皮奥伊州特雷西纳）种质库中的8份P. pyramidalis材料和2份P. bracteosa材料进行了随机扩增多态性DNA分析。使用11条选定的任意序列引物进行扩增反应，产生了167个片段，总体多态性为70.38%。仅在P. pyramidalis材料中产生了5个单态位点，而3个独特的单态位点与P. bracteosa相关，这些代表了潜在的物种特异性标记。Poincianella属材料之间的相似系数较低（平均值为0.59），但变化范围较广（0.443至0.748）。使用非加权配对组方法构建的相似性矩阵和树状图能够将Poincianella属材料分为两个主要类群（由两个不同物种代表），而P. pyramidalis的材料可以进一步分为三个亚组。在Poincianella属中检测到的高水平遗传多样性可用于未来的育种计划以培育改良品种，不过如果从巴西东北部半干旱地区的其他地点收集更多样本，可能会更好地利用这种变异性。
