Luz G A, Gomes S O, Araujo Neto R B, Nascimento M S C B, Lima P S C
Laboratório de Biologia Molecular, Centro de Ciências Agrárias, Universidade Federal do Piauí, Teresina, PI, Brasil.
Laboratório de Biologia Molecular, Embrapa Meio-Norte, Teresina, PI, Brasil.
Genet Mol Res. 2015 Nov 27;14(4):15242-8. doi: 10.4238/2015.November.25.12.
Cratylia argentea (Desv.) Kuntze (Fabaceae) is a drought-tolerant, perennial legume found primarily in Brazil, Bolivia, and Peru. The shrub is well adapted to acid soils and exhibits high productivity and nutritional value, characteristics that would favor its use as a dry season animal forage supplement in semiarid regions. In plant improvement programs, the production of elite hybrids with superior traits is generally achieved by crossing parents that exhibit the highest level of genetic divergence. Therefore, the aim of the present study was to assess genetic diversity among 13 accessions of C. argentea from the same population maintained in the active germplasm bank of Embrapa Meio-Norte using inter-simple sequence repeat (ISSR) markers. Genetic similarities between C. argentea accessions were estimated from Jaccard coefficients, and a dendrogram was constructed using the unweighted pair group method with arithmetic average (UPGMA). The set of 15 primers selected for ISSR analysis generated a total of 313 loci of which 79.23% were polymorphic. The mean number of bands per primer was 20.87, and the amplicons ranged from 280 to 3000 bp in size. Primers UBC834 and UBC827 generated the largest number of polymorphic loci and exhibited 90.91 and 100% polymorphism, respectively. The coefficients of genetic similarity among accessions varied between 0.49 and 0.73. UPGMA cluster analysis allowed the identification of four genotypic groups and demonstrated the existence of considerable variability within the collection. Potential progenitors were selected that would offer good possibilities of obtaining unusual and favorable combinations of genes in a plant breeding program.
银叶克拉蒂亚（Cratylia argentea (Desv.) Kuntze，豆科）是一种耐旱的多年生豆科植物，主要分布在巴西、玻利维亚和秘鲁。这种灌木非常适应酸性土壤，具有高生产力和营养价值，这些特性使其适合作为半干旱地区旱季动物饲料的补充。在植物改良计划中，通常通过杂交具有最高遗传差异水平的亲本，来培育具有优良性状的精英杂种。因此，本研究的目的是利用简单序列重复区间（ISSR）标记，评估巴西农牧业研究公司东北中心（Embrapa Meio-Norte）活性种质库中保存的来自同一群体的13份银叶克拉蒂亚种质的遗传多样性。根据杰卡德系数估算银叶克拉蒂亚种质之间的遗传相似性，并使用非加权算术平均法（UPGMA）构建聚类图。为ISSR分析选择的15组引物共产生了313个位点，其中79.23%为多态性位点。每个引物的平均条带数为20.87，扩增片段大小在280至3000 bp之间。引物UBC834和UBC827产生的多态性位点最多，分别表现出90.91%和100%的多态性。种质之间的遗传相似系数在0.49至0.73之间。UPGMA聚类分析能够识别出四个基因型组，并表明该收集群体中存在相当大的变异性。选择了潜在的亲本，它们在植物育种计划中具有获得不寻常且有利基因组合的良好可能性。
