School of Chemical Engineering, Tianjin University, Tianjin 300072, China.
School of Life Science, Beijing Institute of Technology, Beijing 100081, China.
Bioresour Technol. 2014 Oct;170:38-44. doi: 10.1016/j.biortech.2014.07.063. Epub 2014 Jul 24.
In this study, thermo-tolerant devices consisting of heat shock genes from thermophiles were designed and introduced into Saccharomyces cerevisiae for improving its thermo-tolerance. Among ten engineered thermo-tolerant yeasts, T.te-TTE2469, T.te-GroS2 and T.te-IbpA displayed over 25% increased cell density and 1.5-4-fold cell viability compared with the control. Physiological characteristics of thermo-tolerant strains revealed that better cell wall integrity, higher trehalose content and enhanced metabolic energy were preserved by thermo-tolerant devices. Engineered thermo-tolerant strain was used to investigate the impact of thermo-tolerant device on pathway efficiency by introducing β-amyrin synthesis pathway, showed 28.1% increased β-amyrin titer, 28-35°C broadened growth temperature range and 72h shortened fermentation period. The results indicated that implanting heat shock proteins from thermophiles to S. cerevisiae would be an efficient approach to improve its thermo-tolerance.
在这项研究中，设计并引入了耐热基因工程耐热装置，这些耐热基因来自嗜热微生物，以提高酿酒酵母的耐热性。在十种工程耐热酵母中，T.te-TTE2469、T.te-GroS2 和 T.te-IbpA 的细胞密度比对照提高了 25%以上，细胞活力提高了 1.5-4 倍。耐热菌株的生理特性表明，耐热装置可以保持更好的细胞壁完整性、更高的海藻糖含量和增强的代谢能量。通过引入β-香树脂醇合成途径，工程耐热菌株被用于研究耐热装置对途径效率的影响，结果显示β-香树脂醇产量提高了 28.1%，生长温度范围拓宽到 28-35°C，发酵周期缩短了 72 小时。结果表明，向酿酒酵母中植入来自嗜热微生物的热休克蛋白是提高其耐热性的有效方法。
