1] Marine Microbiology Group, Department of Ecology and Marine Resources, Mediterranean Institute for Advanced Studies (Spanish National Research Council (CSIC)-University of the Balearic Islands (UIB)), E-07190 Esporles, Balearic Islands, Spain. [2] Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany. [3] Ribocon GmbH, Fahrenheitstrasse 1, D-28359 Bremen, Germany.
Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, D-28359 Bremen, Germany.
Nat Rev Microbiol. 2014 Sep;12(9):635-45. doi: 10.1038/nrmicro3330.
Publicly available sequence databases of the small subunit ribosomal RNA gene, also known as 16S rRNA in bacteria and archaea, are growing rapidly, and the number of entries currently exceeds 4 million. However, a unified classification and nomenclature framework for all bacteria and archaea does not yet exist. In this Analysis article, we propose rational taxonomic boundaries for high taxa of bacteria and archaea on the basis of 16S rRNA gene sequence identities and suggest a rationale for the circumscription of uncultured taxa that is compatible with the taxonomy of cultured bacteria and archaea. Our analyses show that only nearly complete 16S rRNA sequences give accurate measures of taxonomic diversity. In addition, our analyses suggest that most of the 16S rRNA sequences of the high taxa will be discovered in environmental surveys by the end of the current decade.
公共可用的小亚基核糖体 RNA 基因序列数据库，在细菌和古菌中也被称为 16S rRNA，其数量正在迅速增长，目前已超过 400 万。然而，对于所有细菌和古菌，尚未存在一个统一的分类和命名框架。在这篇分析文章中，我们基于 16S rRNA 基因序列同一性，为细菌和古菌的高级分类单元提出了合理的分类界限，并为与培养细菌和古菌的分类学兼容的未培养分类单元的划定提出了一个基本原理。我们的分析表明，只有近乎完整的 16S rRNA 序列才能准确衡量分类多样性。此外，我们的分析还表明，到本世纪末，环境调查中将会发现大多数高级分类单元的 16S rRNA 序列。
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