Yang Shang, Liu Guohong, Li Ruili, Yu Wei, Huang Yuefei, Wu Xiaofeng, Zhou Shungui, Li Bing
Water Research Center, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, China.
Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou, 35003, Fujian, People's Republic of China.
Curr Microbiol. 2025 Aug 4;82(9):431. doi: 10.1007/s00284-025-04419-3.
An aerobic phosphorus-recovering strain, designated WS-203, was isolated from mangrove sediment. Strain WS-203 showed the highest 16S rRNA gene sequence similarity to the type strain of Pelagibacterium lacus (98.1%). A phylogenetic tree based on 16S rRNA gene sequences indicated that strain WS-203 clustered with members of the genus Pelagibacterium. Growth of strain WS-203 was observed at 20-37 °C (optimum, 30 °C), pH 6.0-9.5 (optimum, pH 7.5) and with 0-9.0% (w/v) NaCl (optimum, 3.0%). Strain WS-203 contained Q-10 as the respiratory quinone and C ω6c/C ω7c and C ω8c cyclo as the major fatty acids. The polar lipids detected in the strain were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentified lipids and four unidentified glycolipids. The genomic DNA G + C content of strain WS-203 was 62.2%. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain WS-203 and the closely related Pelagibacterium lacus were 78.6% and 21.7%, respectively, which were lower than the cut-off value (ANI 95-96% and dDDH 70%) for prokaryotic species delineation. On the basis of these results, strain WS-203 represents a novel species of the genus Pelagibacterium, for which the name Pelagibacterium mangrovi sp. nov. is proposed. The type strain is WS-203 (= GDMCC 1.4694 = KCTC 8652).
从红树林沉积物中分离出一株好氧性磷回收菌株，命名为WS-203。菌株WS-203与湖泊栖海菌模式菌株的16S rRNA基因序列相似度最高（98.1%）。基于16S rRNA基因序列构建的系统发育树表明，菌株WS-203与栖海菌属成员聚类在一起。在20-37℃（最适温度30℃）、pH 6.0-9.5（最适pH 7.5）和0-9.0%（w/v）NaCl（最适浓度3.0%）条件下观察到菌株WS-203的生长。菌株WS-203含有Q-10作为呼吸醌，以C ω6c/C ω7c和C ω8c环丙烷脂肪酸作为主要脂肪酸。该菌株中检测到的极性脂质有双磷脂酰甘油、磷脂酰甘油、两种未鉴定的磷脂、两种未鉴定的脂质和四种未鉴定的糖脂。菌株WS-203的基因组DNA G+C含量为62.2%。菌株WS-203与密切相关的湖泊栖海菌之间的平均核苷酸一致性（ANI）和数字DNA-DNA杂交（dDDH）值分别为78.6%和21.7%，均低于原核生物物种划分的阈值（ANI 95-96%和dDDH 70%）。基于这些结果，菌株WS-203代表栖海菌属的一个新物种，为此提出新名称红树林栖海菌（Pelagibacterium mangrovi sp. nov.）。模式菌株为WS-203（=GDMCC 1.4694 = KCTC 8652）。
