Su Junhong, Li Jidong, Zheng Haixue, You Yanan, Luo Xuenong, Li Yungang, Li Xueqiang, Ma Xusheng, Li Junjun, Dou Yongxi, Cai Xuepeng
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, PR China.
State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Lanzhou, PR China; School of Agriculture, Ningxia University, Yinchuan, RP China.
PLoS One. 2014 Aug 13;9(8):e104446. doi: 10.1371/journal.pone.0104446. eCollection 2014.
The adjuvant effects of Lactobacillus acidophilus on DNA vaccination are not fully understood. It has been hypothesized that swine-derived Lactobacillus acidophilus SW1 (LASW1) could function as an immune adjuvant to enhance antigen-specific immune responses after foot-and-mouth disease (FMD) DNA vaccination in mice. To evaluate the effect of oral LASW1 on the immune response to a DNA vaccine (pRC/CMV-vp1) harboring FMD VP1 gene, anti-FMDV antibody and its isotypes, T-cell proliferation, and cytokine detection were investigated. The results showed that LASW1 was able to enhance FMDV-specific antibody levels and FMDV-neutralizing antibodies. After a booster vaccine, the anti-FMDV antibody titers and FMDV-neutralizing antibodies levels induced by pRC/CMV-vp1 were higher in mice treated with LSAW1 than in the group immunized with pRC/CMV-vp1 alone (the control). Using T-cell proliferation, the stimulation index of the LASW1 group was significantly higher in response to ConA and 146S antigen (P<0.05) than in the control group. Importantly, higher concentrations of IFN-γ and IFN-γ-producing cells were also observed in splenocytes isolated from the experimental LASW1 mice, indicating that INF-γ secretion is important to the immune response to LASW1. The results indicate that LASW1 is a promising immune adjuvant in DNA vaccination against FMD when administrated orally.
嗜酸乳杆菌对DNA疫苗的佐剂作用尚未完全明确。据推测，猪源嗜酸乳杆菌SW1（LASW1）可作为一种免疫佐剂，增强小鼠口蹄疫（FMD）DNA疫苗接种后的抗原特异性免疫反应。为评估口服LASW1对携带FMD VP1基因的DNA疫苗（pRC/CMV-vp1）免疫反应的影响，研究了抗FMDV抗体及其亚型、T细胞增殖和细胞因子检测情况。结果显示，LASW1能够提高FMDV特异性抗体水平和FMDV中和抗体水平。加强免疫后，用LSAW1处理的小鼠中，pRC/CMV-vp1诱导的抗FMDV抗体滴度和FMDV中和抗体水平高于单独用pRC/CMV-vp1免疫的组（对照组）。通过T细胞增殖实验，LASW1组对ConA和146S抗原反应的刺激指数显著高于对照组（P<0.05）。重要的是，在从实验性LASW1小鼠分离的脾细胞中也观察到更高浓度的IFN-γ和产生IFN-γ的细胞，表明INF-γ分泌对LASW1的免疫反应很重要。结果表明，口服LASW1在FMD DNA疫苗接种中是一种有前景的免疫佐剂。
