Department of Chemistry, Indian Institute of Technology Roorkee , Roorkee 247667, Uttarakhand, India.
J Phys Chem B. 2014 Sep 4;118(35):10543-51. doi: 10.1021/jp5038427. Epub 2014 Aug 21.
The present manuscript reports the characterization, optimization of rheological properties, and loading and release capabilities of 5'-GMP mediated β-FeOOH hydrogel. Circular dichroism (CD) analysis indicates it to contain mainly the left-handed helix similar to that of Z-DNA. The highest viscosity (>300 cP) corresponds to the sample containing 2.5 × 10(-3) mol dm(-3) of 5'-GMP (SP2H). Field emission scanning electron microscope (FESEM) and transmission electron microscope (TEM) studies indicate the freeze-dried (FD) SP2H to be porous in nature, which is also supported by its high Brunauer-Emmett-Teller (BET) surface area of 226 m(2)/g as compared to that of SP3H (75 m(2)/g). Selected area electron diffraction (SAED) analysis and Raman spectroscopy show it to contain β-FeOOH phase. The FD SP2H exhibits the high swelling ratio (326%) and loading capacity for methylene blue (MB) dye. It displays a controlled and efficient release (>90%) for optimized [MB] (2.5 × 10(-4) mol dm(-3)) in 48 h. The low toxicity of as synthesized FD SP2H nanostructures against MDA-MB-231 (breast cancer cells) up to 100 μg/mL suggests its biocompatible nature. The high porosity, surface area, % swelling, and loading and release performance of the hydrogel indicate its potential for drug delivery and other biological applications.
本手稿报告了 5'-GMP 介导的β-FeOOH 水凝胶的特性、流变性优化、载药和释放性能。圆二色性（CD）分析表明，它主要含有类似于 Z-DNA 的左手螺旋。载药量最高（>300 cP）的样品中含有 2.5×10（-3）mol dm（-3）的 5'-GMP（SP2H）。场发射扫描电子显微镜（FESEM）和透射电子显微镜（TEM）研究表明，冷冻干燥（FD）的 SP2H 具有多孔性，其比 SP3H（75 m（2）/g）高的 Brunauer-Emmett-Teller（BET）表面积（226 m（2）/g）也支持这一点。选区电子衍射（SAED）分析和拉曼光谱表明，它含有β-FeOOH 相。FD SP2H 表现出高的溶胀比（326%）和对亚甲蓝（MB）染料的载药量。在 48 小时内，优化的[MB]（2.5×10（-4）mol dm（-3））可以实现>90%的控制和有效的释放。合成的 FD SP2H 纳米结构对 MDA-MB-231（乳腺癌细胞）的低毒性高达 100μg/mL，表明其具有生物相容性。水凝胶的高孔隙率、表面积、溶胀率、载药量和释放性能表明其在药物输送和其他生物应用方面的潜力。
