Hu Xiaohong, Gong Xiao
School of Material Engineering, Jinling Institute of Technology, Nanjing 211169, China.
State Key Laboratory of Silicate Materials for Architectures, Wuhan University of Technology, No. 122, Luoshi Road, Wuhan 430070, China.
J Colloid Interface Sci. 2016 May 15;470:62-70. doi: 10.1016/j.jcis.2016.02.037. Epub 2016 Feb 17.
Hydrogels for drug delivery have attracted extensive interests since they can be used for biomaterials such as contact lenses. Here, we report that biocompatible hydrogels for contact lenses with controlled drug delivery behavior can be fabricated using copolymer hydrogels and Layer-by-Layer (LbL) surface modification technique. Methyl acrylic anhydride (MAA) modified β-cyclodextrin (β-CD) (MA-β-CD) was synthesized and copolymerized with hydroxyethyl methacrylate (HEMA) to form copolymer hydrogel. The introduction of second monomer of MA-β-CD would accelerate the polymerization of hydrogel, leading to increase of residual CC groups. The structure of copolymers was characterized by differential scanning calorimetry (DSC). Transparence, equilibrium swelling ratio and contact angle of copolymer hydrogel were also detailed discussed in the work. In vitro drug release results showed that copolymer hydrogel with higher MA-β-CD content exhibited a better drug loading capacity and drug release behaviors could be tuned by MA-β-CD/monomer ratio. Finally, alkynyl functional hyaluronic acid (HA-BP) and nitrine functional chitosan (CS-N3) were synthesized and covalently cross-linked to copolymer hydrogel surface using LbL technique through click chemistry. The successful LbL multilayers were confirmed by X-ray Photoelectron Spectroscopy (XPS). Resultsofcytotoxicityexperiment revealed that the hydrogels were biocompatible since they could support the growth of cells.
用于药物递送的水凝胶因其可用于隐形眼镜等生物材料而引起了广泛关注。在此，我们报道了一种可通过共聚物水凝胶和层层（LbL）表面改性技术制备的具有可控药物递送行为的隐形眼镜用生物相容性水凝胶。合成了甲基丙烯酸酐（MAA）改性的β-环糊精（β-CD）（MA-β-CD），并将其与甲基丙烯酸羟乙酯（HEMA）共聚以形成共聚物水凝胶。MA-β-CD第二单体的引入会加速水凝胶的聚合，导致残余CC基团增加。通过差示扫描量热法（DSC）对共聚物的结构进行了表征。在这项工作中还详细讨论了共聚物水凝胶的透明度、平衡溶胀率和接触角。体外药物释放结果表明，MA-β-CD含量较高的共聚物水凝胶表现出更好的载药能力，药物释放行为可通过MA-β-CD/单体比例进行调节。最后，合成了炔基功能化透明质酸（HA-BP）和硝胺功能化壳聚糖（CS-N3），并通过点击化学利用LbL技术将它们共价交联到共聚物水凝胶表面。通过X射线光电子能谱（XPS）证实了成功制备的LbL多层膜。细胞毒性实验结果表明，这些水凝胶具有生物相容性，因为它们能够支持细胞生长。
