Álvarez-Díaz Pablo David, Ruiz Jesús, Arbib Zouhayr, Barragán Jesús, Garrido-Pérez Carmen, Perales José Antonio
Environmental Technologies Department, Andalusian Center of Science and Marine Technology (CACYTMAR), Universidad de Cádiz, Campus of Puerto Real, 11510, Puerto Real, Cádiz, Spain.
Chiclana Natural S.A.M., Plaza de España S/N, 11130, Chiclana, Cádiz, Spain.
Appl Biochem Biotechnol. 2014 Oct;174(4):1471-1483. doi: 10.1007/s12010-014-1126-5. Epub 2014 Aug 14.
The aim of this work was to study the stimulation of lipid production on the microalga Ankistrodesmus falcatus by varying cultivation conditions during the stationary phase. The effect of three factors (presence and absence of nitrogen, phosphorus, and light) has been tested once the cultures reached the stationary phase with the aim to increase the value of the biomass for further applications. Lipid content, elemental composition, Nile red fluorescence evolution, and calorific value of microalgal biomass were studied as well as biomass growth. Biomass presented a lipid content of 36.54 % at the end of the first stage, while at the end of the second stage, the experiments with the absence of phosphorus increased their lipid content until 45.94 and 44.55 %, the first with nitrogen and light presence and the second with absence of all factors. The combination of phosphorus absence and nitrogen and light presence achieved the highest lipid productivity (20.27 mg/L/day). The two-stage strategy to culture microalgae is a feasible option to increase the economic or energetic value of biomass.
这项工作的目的是研究在稳定期通过改变培养条件对微藻镰形纤维藻脂质产生的刺激作用。一旦培养物达到稳定期，就测试了三个因素（氮、磷和光的存在与否）的影响，目的是提高生物质的价值以用于进一步应用。研究了微藻生物质的脂质含量、元素组成、尼罗红荧光演变、热值以及生物质生长情况。在第一阶段结束时，生物质的脂质含量为36.54%，而在第二阶段结束时，无磷实验将其脂质含量提高到了45.94%和44.55%，第一个是在有氮和光的情况下，第二个是在所有因素都不存在的情况下。无磷与有氮和光的组合实现了最高的脂质生产率（20.27毫克/升/天）。两阶段培养微藻的策略是提高生物质经济或能源价值的可行选择。
