Dy Grace K, Ylagan Lourdes, Pokharel Saraswati, Miller Austin, Brese Elizabeth, Bshara Wiam, Morrison Carl, Cance William G, Golubovskaya Vita M
*Department of Medicine; †Department of Pathology; ‡Department of Biostatistics; and §Department of Surgical Oncology, Roswell Park Cancer Institute, Buffalo, NY.
J Thorac Oncol. 2014 Sep;9(9):1278-84. doi: 10.1097/JTO.0000000000000248.
Focal adhesion kinase (FAK) plays a significant role in cancer cell survival signaling and is overexpressed in various malignancies, including lung cancer. Previous studies suggest that FAK overexpression is an independent factor predicting poor prognosis in non-small-cell lung cancer (NSCLC). The aim of this study is to confirm these findings specifically in stage I NSCLC.
A retrospective tissue microarray (TMA) analysis of FAK protein expression by immunohistochemistry was performed in 157 surgically resected stage I NSCLC specimen and in the corresponding matched normal lung tissue. The FAK 4.47 monoclonal antibody was used for FAK immunostaining. The scoring system of triplicate tumor cores included intensity of staining plus extent of staining for a composite score that ranged from 0 to 6. The association between FAK score and survival was evaluated.
There were 103 stage IA and 54 stage IB patients, with mean follow-up of 5.5 years. Normal lung alveoli and interstitial tissue had mean FAK score of 0 (median score 0, range 0 to 2). Tumor samples had mean FAK score 3.1 (median score 3.5, range 0-6), with 57% of the samples having FAK score ≥ 3. Continuous FAK score was not associated with demographic data, tumor histology, or grade, nor survival in this cohort of stage I NSCLC patients.
FAK is expressed in more than 50% of stage I NSCLC lung cancer but not in normal lung alveoli and interstitial tissue. FAK expression is not associated with survival outcome in this North American cohort.
粘着斑激酶（FAK）在癌细胞存活信号传导中起重要作用，并且在包括肺癌在内的各种恶性肿瘤中过表达。先前的研究表明，FAK过表达是预测非小细胞肺癌（NSCLC）预后不良的独立因素。本研究的目的是在I期NSCLC中具体证实这些发现。
对157例手术切除的I期NSCLC标本及相应匹配的正常肺组织进行免疫组织化学法检测FAK蛋白表达的回顾性组织微阵列（TMA）分析。使用FAK 4.47单克隆抗体进行FAK免疫染色。对三个肿瘤核心的评分系统包括染色强度加上染色范围，综合评分为0至6分。评估FAK评分与生存率之间的关联。
有103例IA期和54例IB期患者，平均随访5.5年。正常肺泡和间质组织的FAK平均评分为0（中位数为0，范围为0至2）。肿瘤样本的FAK平均评分为3.1（中位数为3.5，范围为0 - 6），57%的样本FAK评分≥3。在这组I期NSCLC患者中，连续的FAK评分与人口统计学数据、肿瘤组织学或分级以及生存率均无关联。
超过50%的I期NSCLC肺癌中表达FAK，但正常肺泡和间质组织中不表达。在这个北美队列中，FAK表达与生存结果无关。
