小细胞肺癌中粘着斑激酶的表达。
Expression of focal adhesion kinase in small-cell lung carcinoma.
机构信息
Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University, Nashville, Tennessee 37232-6838, USA.
出版信息
Cancer. 2012 Mar 1;118(5):1293-301. doi: 10.1002/cncr.26382. Epub 2011 Jul 28.
BACKGROUND
The focal adhesion kinase (FAK) is a non-receptor tyrosine kinase linked to tumor growth, invasion, and metastasis. FAK is overexpressed and associated with prognosis in many cancers, but its prognostic value in small-cell lung carcinoma (SCLC) is unknown and was the focus of this study.
METHODS
Total FAK expression was analyzed via immunohistochemistry in tissue microarrays consisting of formalin-fixed, paraffin-embedded SCLC specimens from 85 patients. FAK staining scores were tested for correlations with pathological characteristics and clinical outcomes. Phospho-paxillin was also tested in 35 of the 85 specimens to evaluate whether FAK expression was associated with downstream signaling.
RESULTS
Specific FAK expression was localized to the cytoplasm of 78/85 (92%) SCLCs. FAK expression was scored low in 11 (13%), moderate in 17 (20%), and high in 50 (59%) SCLCs. FAK staining scores treated as continuous variables did not correlate with SCLC disease stage, response to therapy, recurrence/progression-free survival, or overall survival. Moreover, total FAK expression did not correlate with phospho-paxillin Tyr(118) expression.
CONCLUSIONS
Total FAK is strongly expressed in a majority of SCLC tumors. However, the expression evaluated via immunohistochemistry is not a prognostic factor in patients with SCLC.
背景
粘着斑激酶（FAK）是一种与肿瘤生长、侵袭和转移相关的非受体酪氨酸激酶。FAK 在许多癌症中过度表达并与预后相关，但它在小细胞肺癌（SCLC）中的预后价值尚不清楚，这也是本研究的重点。
方法
通过免疫组织化学分析组织微阵列中 85 例福尔马林固定、石蜡包埋的 SCLC 标本中总 FAK 的表达。FAK 染色评分与病理特征和临床结果进行相关性检验。还对 85 例标本中的 35 例进行磷酸化桩蛋白检测，以评估 FAK 表达是否与下游信号转导相关。
结果
78/85（92%）例 SCLC 中细胞质内有特异的 FAK 表达。FAK 表达评分低 11 例（13%），中 17 例（20%），高 50 例（59%）。FAK 染色评分作为连续变量与 SCLC 疾病分期、对治疗的反应、无复发/进展生存或总生存均无相关性。此外，总 FAK 表达与磷酸化桩蛋白 Tyr（118）表达无相关性。
结论
大多数 SCLC 肿瘤中均强烈表达总 FAK。然而，通过免疫组织化学评估的表达不是 SCLC 患者的预后因素。
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