Division of Allergy, Pulmonary, and Critical Care Medicine, Vanderbilt University, Nashville, Tennessee 37232-6838, USA.
Cancer. 2012 Mar 1;118(5):1293-301. doi: 10.1002/cncr.26382. Epub 2011 Jul 28.
The focal adhesion kinase (FAK) is a non-receptor tyrosine kinase linked to tumor growth, invasion, and metastasis. FAK is overexpressed and associated with prognosis in many cancers, but its prognostic value in small-cell lung carcinoma (SCLC) is unknown and was the focus of this study.
Total FAK expression was analyzed via immunohistochemistry in tissue microarrays consisting of formalin-fixed, paraffin-embedded SCLC specimens from 85 patients. FAK staining scores were tested for correlations with pathological characteristics and clinical outcomes. Phospho-paxillin was also tested in 35 of the 85 specimens to evaluate whether FAK expression was associated with downstream signaling.
Specific FAK expression was localized to the cytoplasm of 78/85 (92%) SCLCs. FAK expression was scored low in 11 (13%), moderate in 17 (20%), and high in 50 (59%) SCLCs. FAK staining scores treated as continuous variables did not correlate with SCLC disease stage, response to therapy, recurrence/progression-free survival, or overall survival. Moreover, total FAK expression did not correlate with phospho-paxillin Tyr(118) expression.
Total FAK is strongly expressed in a majority of SCLC tumors. However, the expression evaluated via immunohistochemistry is not a prognostic factor in patients with SCLC.
粘着斑激酶（FAK）是一种与肿瘤生长、侵袭和转移相关的非受体酪氨酸激酶。FAK 在许多癌症中过度表达并与预后相关，但它在小细胞肺癌（SCLC）中的预后价值尚不清楚，这也是本研究的重点。
通过免疫组织化学分析组织微阵列中 85 例福尔马林固定、石蜡包埋的 SCLC 标本中总 FAK 的表达。FAK 染色评分与病理特征和临床结果进行相关性检验。还对 85 例标本中的 35 例进行磷酸化桩蛋白检测，以评估 FAK 表达是否与下游信号转导相关。
78/85（92%）例 SCLC 中细胞质内有特异的 FAK 表达。FAK 表达评分低 11 例（13%），中 17 例（20%），高 50 例（59%）。FAK 染色评分作为连续变量与 SCLC 疾病分期、对治疗的反应、无复发/进展生存或总生存均无相关性。此外，总 FAK 表达与磷酸化桩蛋白 Tyr（118）表达无相关性。
大多数 SCLC 肿瘤中均强烈表达总 FAK。然而，通过免疫组织化学评估的表达不是 SCLC 患者的预后因素。
