一种新型的GTP结合蛋白Sar1p参与从内质网到高尔基体的转运。
A novel GTP-binding protein, Sar1p, is involved in transport from the endoplasmic reticulum to the Golgi apparatus.
作者信息
Nakańo A, Muramatsu M
机构信息
Department of Biology, Faculty of Science, University of Tokyo, Japan.
出版信息
J Cell Biol. 1989 Dec;109(6 Pt 1):2677-91. doi: 10.1083/jcb.109.6.2677.
Abstract
SAR1, a gene that has been isolated as a multicopy suppressor of the yeast ER-Golgi transport mutant sec12, encodes a novel GTP-binding protein. Its nucleotide sequence predicts a 21-kD polypeptide that contains amino acid sequences highly homologous to GTP-binding domains of many ras-related proteins. Gene disruption experiments show that SAR1 is essential for cell growth. To test its function further, SAR1 has been placed under control of the GAL1 promoter and introduced into a haploid cell that had its chromosomal SAR1 copy disrupted. This mutant grows normally in galactose medium but arrests growth 12-15 h after transfer to glucose medium. At the same time, mutant cells accumulate ER precursor forms of a secretory pheromone, alpha-mating factor, and a vacuolar enzyme, carboxypeptidase Y. We propose that Sec12p and Sarlp collaborate in directing ER-Golgi protein transport.
摘要
SAR1是一个作为酵母内质网-高尔基体转运突变体sec12的多拷贝抑制子而被分离出来的基因，它编码一种新型的GTP结合蛋白。其核苷酸序列预测有一个21-kD的多肽，该多肽含有与许多ras相关蛋白的GTP结合结构域高度同源的氨基酸序列。基因破坏实验表明，SAR1对细胞生长至关重要。为了进一步测试其功能，已将SAR1置于GAL1启动子的控制之下，并导入一个其染色体上的SAR1拷贝已被破坏的单倍体细胞中。该突变体在半乳糖培养基中正常生长，但在转移到葡萄糖培养基后12 - 15小时停止生长。与此同时，突变细胞积累了分泌性信息素α-交配因子和液泡酶羧肽酶Y的内质网前体形式。我们提出，Sec12p和Sarlp在指导内质网-高尔基体蛋白转运过程中相互协作。
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J Cell Biol. 1989 Dec;109(6 Pt 1):2677-91. doi: 10.1083/jcb.109.6.2677.
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