真核生物核糖体循环因子ABCE1在黑腹果蝇细胞无义mRNA降解中的功能作用。
A functional involvement of ABCE1, eukaryotic ribosome recycling factor, in nonstop mRNA decay in Drosophila melanogaster cells.
作者信息
Kashima Isao, Takahashi Masaki, Hashimoto Yoshifumi, Sakota Eri, Nakamura Yoshikazu, Inada Toshifumu
机构信息
The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai 980-8578, Japan.
The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
出版信息
Biochimie. 2014 Nov;106:10-6. doi: 10.1016/j.biochi.2014.08.001. Epub 2014 Aug 14.
When ribosomes encounter mRNAs lacking stop codons, two quality-control machineries, NSD for nonstop mRNA decay and ribosome quality control (RQC) for co-translational degradation of the nonstop protein by the proteasome, are triggered to eliminate aberrant molecules. In yeast, it is known that Dom34 (a homolog of eRF1) and Ltn1 (an E3 ubiquitin ligase) play crucial roles in NSD and RQC, respectively, by triggering ribosome rescue at the 3' end of nonstop mRNAs and proteasome-dependent polypeptide degradation. Here we confirmed the essential role of Ltn1 in RQC for nonstop products in Drosophila cells, and further uncovered a functional role of ABCE1, a eukaryotic ribosome recycling factor, in NSD in Drosophila cells.
当核糖体遇到缺乏终止密码子的mRNA时，两种质量控制机制会被触发以消除异常分子，即用于无终止mRNA降解的NSD和用于蛋白酶体对无终止蛋白质进行共翻译降解的核糖体质量控制（RQC）。在酵母中，已知Dom34（eRF1的同源物）和Ltn1（一种E3泛素连接酶）分别在NSD和RQC中发挥关键作用，通过在无终止mRNA的3'端触发核糖体拯救和蛋白酶体依赖性多肽降解。在这里，我们证实了Ltn1在果蝇细胞中对无终止产物的RQC中的重要作用，并进一步揭示了真核核糖体回收因子ABCE1在果蝇细胞NSD中的功能作用。
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