Balto Hanan A, Al-Manei Khaled K, Bin-Mohareb Talal M, Shakoor Zahid A, Al-Hadlaq Solaiman M
Department of Restorative Dental Science, Division of Endodontics, College of Dentistry, King Saud University, PO Box 62645, Riyadh 11545, Kingdom of Saudi Arabia. Tel. +966 (11) 4677420. E-mail.
Saudi Med J. 2014 Aug;35(8):810-5.
To assess the cytotoxic potential of Salvadora persica (S. persica) extracts on human gingival fibroblast (HGF) cells.
This study was conducted between January and May 2012 in collaboration with Dental Caries Research Chair, College of Dentistry, King Saud University, Riyadh, Saudi Arabia. Extracts of S. persica using hexane, ethylacetate, and ethanol as solvents at concentrations of 0.5 mg/ml and 1 mg/ml were evaluated for their cytotoxic activity against HGFs using the 3 cytotoxic assays: (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole) (MTS), lactic dehydrogenase (LDH), and crystal violet (CV). International standards for the evaluation of medical materials recommended cut-off value of cell survival >70% was used for interpretation of the results.
Ethanol extract of S. persica at 0.5 mg/ml and 1 mg/ml and hexane extract of S. persica at 0.5 mg/ml were completely devoid of cytotoxic activity, hexane extract at 1 mg/ml in comparison with controls demonstrated some cytotoxicity with cell survival of 88% (p=0.045) in MTS, 86% (p=0.01) in LDH, and 88% (p=0.002) in CV assays. Similarly, ethyl acetate extract of S. persica at 0.5 mg/ml maintained cell viability of 91% in MTS, 81% in LDH, and 80% in CV assays. Maximum cytotoxicity against HGFs was observed with ethyl acetate extract of S. persica at 1 mg/ml with cell survival of 60% in MTS, 40% in LDH, and 66% CV assays (p=0.0001).
The acceptable level of cytotoxicity associated with S. persica ethanol and hexane extracts requires further evaluation to be used as irrigation solutions in endodontic treatment.
评估桃金娘科植物没药提取物对人牙龈成纤维细胞（HGF）的细胞毒性潜力。
本研究于2012年1月至5月与沙特阿拉伯利雅得国王沙特大学牙科学院龋齿研究主席合作开展。使用己烷、乙酸乙酯和乙醇作为溶剂，浓度分别为0.5mg/ml和1mg/ml的没药提取物，通过三种细胞毒性试验评估其对HGFs的细胞毒性活性：（3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐，一种四氮唑）（MTS）、乳酸脱氢酶（LDH）和结晶紫（CV）。用于评估医用材料的国际标准推荐细胞存活率>70%的临界值用于结果解释。
浓度为0.5mg/ml和1mg/ml的没药乙醇提取物以及浓度为0.5mg/ml的没药己烷提取物完全没有细胞毒性活性，浓度为1mg/ml的己烷提取物与对照组相比，在MTS试验中细胞存活率为88%（p = 0.045），在LDH试验中为86%（p = 0.01），在CV试验中为88%（p = 0.002），显示出一定的细胞毒性。同样，浓度为0.5mg/ml的没药乙酸乙酯提取物在MTS试验中细胞活力维持在91%，在LDH试验中为81%，在CV试验中为80%。在浓度为1mg/ml的没药乙酸乙酯提取物中观察到对HGFs的最大细胞毒性，在MTS试验中细胞存活率为60%，在LDH试验中为40%，在CV试验中为66%（p = 0.0001）。
与没药乙醇和己烷提取物相关的可接受细胞毒性水平需要进一步评估，以用作牙髓治疗中的冲洗液。
