Albabtain Reham, Azeem Muhammad, Wondimu Zenebech, Lindberg Tulay, Borg-Karlson Anna Karin, Gustafsson Anders
Department of Dental Medicine, Unit of Periodontology, Karolinska Institutet, P.O. Box 4064, 141 04 Stockholm, Sweden.
Department of Dental Hygienist, Medical Sciences College, King Saud University, Riyadh, Saudi Arabia.
Evid Based Complement Alternat Med. 2017;2017:2576548. doi: 10.1155/2017/2576548. Epub 2017 Apr 18.
is commonly used chewing sticks in many parts of the world as an oral hygiene tool. This study measured the amount of benzyl isothiocyanate (BITC) released into the mouth and assessed its retention time in saliva. The study also tested if the released amount of BITC could potentially be antibacterial or cytotoxic. Twelve subjects brushed their teeth with fresh Miswak once, twice, and four times. The amount of BITC in the saliva and in the used brushes was quantified using gas chromatography-mass spectrometry. The antibacterial effect of BITC and Miswak essential oil (MEO) was tested against , and . The cytotoxic effect on gingival fibroblasts and keratinocytes was tested using MTT. The highest amount of the active compounds was detected in saliva after using the Miswak tip for once and immediately. It significantly decreased when the Miswak tip was used more than once and thus after 10 min. The growth of the tested bacteria was inhibited by MEO and BITC in a dose dependent manner, being the most sensitive. MTT assay showed that BITC and MEO were cytotoxic towards gingival fibroblasts while oral keratinocytes showed resistance. This study suggests that the Miswak tip should be cut before each use to ensure the maximum effect.
在世界许多地方，它是常用的咀嚼棒，作为一种口腔卫生工具。本研究测量了释放到口腔中的异硫氰酸苄酯（BITC）的量，并评估了其在唾液中的保留时间。该研究还测试了释放的BITC量是否可能具有抗菌或细胞毒性。12名受试者分别用新鲜的牙刷一次、两次和四次刷牙。使用气相色谱-质谱法定量唾液和用过的牙刷中BITC的量。测试了BITC和牙刷精油（MEO）对 、 和 的抗菌作用。使用MTT测试对牙龈成纤维细胞和角质形成细胞的细胞毒性作用。使用牙刷尖端一次并立即后，唾液中检测到的活性化合物量最高。当使用牙刷尖端超过一次并因此在10分钟后，其显著下降。MEO和BITC以剂量依赖性方式抑制测试细菌的生长， 最敏感。MTT分析表明，BITC和MEO对牙龈成纤维细胞具有细胞毒性，而口腔角质形成细胞则具有抗性。本研究表明，每次使用前应切割牙刷尖端以确保最大效果。
