子宫平滑肌的非钙依赖性收缩
Ca2+-independent contraction of uterine smooth muscle.
作者信息
Matsuo K, Gokita T, Karibe H, Uchida M K
机构信息
Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.
出版信息
Biochem Biophys Res Commun. 1989 Dec 15;165(2):722-7. doi: 10.1016/s0006-291x(89)80026-9.
Rat uterine smooth muscle shows sustained contraction to oxytocin in Ca2+-free medium with EGTA, that is called "Ca-free contraction"(1). Participation of the rise in cytosolic free Ca2+ in this Ca-free contraction was tested. In Ca-free contraction, the cytosolic free Ca2+ level was not changed at all as measured with fura-2. Further, the chelation of cytosolic free Ca2+ with quin-2 did not at all affect Ca-free contraction. These results strongly suggest that Ca-free contraction is not triggered by Ca2+.
在含有乙二醇双（2-氨基乙基醚）四乙酸（EGTA）的无钙培养基中，大鼠子宫平滑肌对催产素表现出持续收缩，这种收缩被称为“无钙收缩”(1)。本研究检测了细胞溶质游离钙离子（Ca2+）浓度升高在这种无钙收缩中的作用。在无钙收缩过程中，用fura-2测量发现细胞溶质游离Ca2+水平完全没有变化。此外，用quin-2螯合细胞溶质游离Ca2+对无钙收缩完全没有影响。这些结果有力地表明，无钙收缩不是由Ca2+触发的。
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