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口腔鳞状细胞癌患者中FADD和FAS基因的甲基化及mRNA表达状态分析

Analysis of methylation and mRNA expression status of FADD and FAS genes in patients with oral squamous cell carcinoma.

作者信息

Saberi Eshaghali, Kordi-Tamandani Dor-Mohammad, Jamali Sara, Rigi-Ladiz Mohammad-Ayoub

机构信息

Department of Biology, University of Sistan and Baluchestan, Zahedan, PO Box 98155-987, Iran,

出版信息

Med Oral Patol Oral Cir Bucal. 2014 Nov 1;19(6):e562-8. doi: 10.4317/medoral.19805.

DOI:10.4317/medoral.19805
PMID:25129245
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4259371/
Abstract

BACKGROUND

Apoptosis is an important mechanism that is responsible for the physiological deletion of harmful, damaged, or unwanted cells. Changed expression of apoptosis-related genes may lead to abnormal cell proliferation and finally to tumor genesis. Our aims were to analyze the promoter methylation and gene expression profiles of FADD and FAS genes in risk of OSCC.

MATERIAL AND METHODS

we analyze the promoter methylation status of FADD and FAS genes using Methylation - Specific PCR (MSP) in 86 OSCC tissues were kept in paraffin and 68 normal oral tissues applied as control. Also, FADD and FAS genes expression were analyzed in 19 cases and 20 normal specimens by Real-Time Reverse-Transcripts PCR.

RESULTS

Aberrant promoter methylation of FADD and FAS genes were detected in 12.79 % (11 of 86) and 60.46 % (52 of 86) of the OSCC cases, respectively, with a significant difference between cases and healthy controls for both FADD and FAS genes (P < 0.001). The gene expression analysis showed statistically significant difference between cases and healthy controls for both FADD (p<0.02) and FAS (p<0.007) genes.

CONCLUSIONS

To the best our knowledge, the data of this study are the first report regarding, the effect of promoter hypermethylation of the FADD and FAS genes in development of OSCC. To confirm the data, it is recommended doing further study in large sample sizes in various genetic populations.

摘要

背景

细胞凋亡是一种重要机制，负责对有害、受损或不需要的细胞进行生理性清除。凋亡相关基因表达的改变可能导致细胞异常增殖，最终引发肿瘤发生。我们的目的是分析FADD和FAS基因的启动子甲基化及基因表达谱在口腔鳞状细胞癌（OSCC）风险中的作用。

材料与方法

我们采用甲基化特异性PCR（MSP）分析86例石蜡包埋的OSCC组织及68例正常口腔组织（作为对照）中FADD和FAS基因的启动子甲基化状态。同时，通过实时逆转录PCR分析19例OSCC病例和20例正常标本中FADD和FAS基因的表达。

结果

在OSCC病例中，分别有12.79%（86例中的11例）和60.46%（86例中的52例）检测到FADD和FAS基因启动子异常甲基化，FADD和FAS基因在病例组和健康对照组之间均存在显著差异（P < 0.001）。基因表达分析显示，FADD（p<0.02）和FAS（p<0.007）基因在病例组和健康对照组之间均存在统计学显著差异。

结论

据我们所知，本研究的数据是关于FADD和FAS基因启动子高甲基化在OSCC发生中的作用的首次报道。为证实这些数据，建议在不同遗传人群中进行大样本量的进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/6659586d4cfc/medoral-19-e562-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/19fa8f683628/medoral-19-e562-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/9c489ef15f3f/medoral-19-e562-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/6659586d4cfc/medoral-19-e562-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/19fa8f683628/medoral-19-e562-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/9c489ef15f3f/medoral-19-e562-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3182/4259371/6659586d4cfc/medoral-19-e562-g003.jpg

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